"A Disposable Protein A-based Immunosensor For Flow Injection Assay With Electrochemical Detection"
Anal. Chim. Acta
2000 Volume 404, Issue 2 Pages 187-194
Notice (8): Undefined variable: uid [APP/View/Elements/citation.ctp, line 40]Code Context ?>
<?php
if($uid!='') {
$viewFile = '/home/stuchalk/public_html/fad/app/View/Elements/citation.ctp'
$dataForView = array(
'data' => array(
'Technique' => array(
'id' => '0543',
'label' => 'Electrode',
'level1' => 'Electrode',
'level2' => 'graphite/polystyrene',
'level3' => '',
'level4' => '',
'level5' => '',
'synonyms' => '',
'champ' => '',
'total' => '1',
'updated' => '0000-00-00 00:00:00',
'name' => 'Electrode, graphite/polystyrene',
'nametotal' => 'Electrode, graphite/polystyrene**1',
'first' => 'E'
),
'Citation' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'c' => array(
'id' => '002240',
'authors' => 'Valat, C.;Limoges, B.;Huet, D.;Romette, J.L.',
'authorsweb' => 'Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette',
'title' => 'A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection',
'journal' => 'Anal. Chim. Acta',
'journal_id' => '0584',
'fadid' => 'ANCA2000V0404P00187',
'year' => '2000',
'volume' => '404',
'issue' => '2',
'startpage' => '187',
'endpage' => '194',
'type' => 'Journal Article',
'analytes' => ';1247;',
'matrices' => '',
'techniques' => ';0400;0209;0543;0269;0431;',
'keywords' => ';0043;0207;',
'abstract' => 'Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG(1) or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02 µg ml-1) and a total assay time of 19 min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow injection analysis, and disposable when necessary.',
'language' => 'English',
'updated' => '2020-12-28 11:25:15',
'sjccheck' => 'Yes',
'sjccheckdate' => '2002-03-30 13:00:00',
'hits' => '11',
'urlcheck' => '2014-10-11 16:33:07',
'urlcheckcode' => 'HTTP/1.1 302 Found',
'pauthor_id' => '01260',
'pauthor' => '!Romette, J.L.',
'address' => 'pau',
'email' => 'pau',
'notes' => null,
'url' => '10.1016/S0003-2670(99)00718-7',
'urltype' => 'doi',
'gotpdf' => 'yes',
'partial' => 'no',
'notanalyte' => '',
'citation' => ''A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection', Anal. Chim. Acta, 2000 404(2) 187-194',
'firstchar' => 'A',
'twochars' => 'A ',
'CitationsTechnique' => array(
'id' => '000364',
'citation_id' => '002240',
'technique_id' => '0543'
),
'Analyte' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Matrix' => array(),
'Keyword' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
)
),
'i' => (int) 0
)
$data = array(
'Technique' => array(
'id' => '0543',
'label' => 'Electrode',
'level1' => 'Electrode',
'level2' => 'graphite/polystyrene',
'level3' => '',
'level4' => '',
'level5' => '',
'synonyms' => '',
'champ' => '',
'total' => '1',
'updated' => '0000-00-00 00:00:00',
'name' => 'Electrode, graphite/polystyrene',
'nametotal' => 'Electrode, graphite/polystyrene**1',
'first' => 'E'
),
'Citation' => array(
(int) 0 => array(
'id' => '002240',
'authors' => 'Valat, C.;Limoges, B.;Huet, D.;Romette, J.L.',
'authorsweb' => 'Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette',
'title' => 'A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection',
'journal' => 'Anal. Chim. Acta',
'journal_id' => '0584',
'fadid' => 'ANCA2000V0404P00187',
'year' => '2000',
'volume' => '404',
'issue' => '2',
'startpage' => '187',
'endpage' => '194',
'type' => 'Journal Article',
'analytes' => ';1247;',
'matrices' => '',
'techniques' => ';0400;0209;0543;0269;0431;',
'keywords' => ';0043;0207;',
'abstract' => 'Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG(1) or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02 µg ml-1) and a total assay time of 19 min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow injection analysis, and disposable when necessary.',
'language' => 'English',
'updated' => '2020-12-28 11:25:15',
'sjccheck' => 'Yes',
'sjccheckdate' => '2002-03-30 13:00:00',
'hits' => '11',
'urlcheck' => '2014-10-11 16:33:07',
'urlcheckcode' => 'HTTP/1.1 302 Found',
'pauthor_id' => '01260',
'pauthor' => '!Romette, J.L.',
'address' => 'pau',
'email' => 'pau',
'notes' => null,
'url' => '10.1016/S0003-2670(99)00718-7',
'urltype' => 'doi',
'gotpdf' => 'yes',
'partial' => 'no',
'notanalyte' => '',
'citation' => ''A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection', Anal. Chim. Acta, 2000 404(2) 187-194',
'firstchar' => 'A',
'twochars' => 'A ',
'CitationsTechnique' => array(
[maximum depth reached]
),
'Analyte' => array(
[maximum depth reached]
),
'Matrix' => array([maximum depth reached]),
'Keyword' => array(
[maximum depth reached]
)
)
)
)
$c = array(
'id' => '002240',
'authors' => 'Valat, C.;Limoges, B.;Huet, D.;Romette, J.L.',
'authorsweb' => 'Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette',
'title' => 'A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection',
'journal' => 'Anal. Chim. Acta',
'journal_id' => '0584',
'fadid' => 'ANCA2000V0404P00187',
'year' => '2000',
'volume' => '404',
'issue' => '2',
'startpage' => '187',
'endpage' => '194',
'type' => 'Journal Article',
'analytes' => ';1247;',
'matrices' => '',
'techniques' => ';0400;0209;0543;0269;0431;',
'keywords' => ';0043;0207;',
'abstract' => 'Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG(1) or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02 µg ml-1) and a total assay time of 19 min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow injection analysis, and disposable when necessary.',
'language' => 'English',
'updated' => '2020-12-28 11:25:15',
'sjccheck' => 'Yes',
'sjccheckdate' => '2002-03-30 13:00:00',
'hits' => '11',
'urlcheck' => '2014-10-11 16:33:07',
'urlcheckcode' => 'HTTP/1.1 302 Found',
'pauthor_id' => '01260',
'pauthor' => '!Romette, J.L.',
'address' => 'pau',
'email' => 'pau',
'notes' => null,
'url' => '10.1016/S0003-2670(99)00718-7',
'urltype' => 'doi',
'gotpdf' => 'yes',
'partial' => 'no',
'notanalyte' => '',
'citation' => ''A disposable Protein A-based immunosensor for flow injection assay with electrochemical detection', Anal. Chim. Acta, 2000 404(2) 187-194',
'firstchar' => 'A',
'twochars' => 'A ',
'CitationsTechnique' => array(
'id' => '000364',
'citation_id' => '002240',
'technique_id' => '0543'
),
'Analyte' => array(
(int) 0 => array(
'id' => '01247',
'name' => 'Immunoglobulin G, mouse',
'iupac_name' => '',
'casrn' => '',
'synonyms' => '',
'total' => '4',
'inchi' => '',
'inchikey' => '',
'formula' => '',
'oxstate' => '',
'url' => '',
'charge' => null,
'class1' => 'Biological',
'class2' => 'NA',
'class3' => 'NA',
'class4' => 'Molecule',
'class5' => '',
'isgroup' => 'no',
'checked' => 'yes',
'citation_count' => '0',
'updated' => '2015-12-11 09:54:00',
'first' => 'I',
'nametotal' => 'Immunoglobulin G, mouse**4',
'AnalytesCitation' => array(
[maximum depth reached]
)
)
),
'Matrix' => array(),
'Keyword' => array(
(int) 0 => array(
'id' => '0043',
'type' => 'Manifold component',
'keyword' => 'Apparatus',
'newKeyword' => '',
'synonyms' => '',
'fao' => '',
'total' => '466',
'first' => 'A',
'keytotal' => 'Apparatus**466',
'CitationsKeyword' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '0207',
'type' => 'Immobilized substance',
'keyword' => 'Immobilized protein',
'newKeyword' => '',
'synonyms' => '',
'fao' => '',
'total' => '8',
'first' => 'I',
'keytotal' => 'Immobilized protein**8',
'CitationsKeyword' => array(
[maximum depth reached]
)
)
)
)
$i = (int) 0
$path = ''
$a = ''
$url = 'http://dx.doi.org/10.1016/S0003-2670(99)00718-7'
$aus = 'Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette'
include - APP/View/Elements/citation.ctp, line 40
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Techniques/view.ctp, line 52
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 968
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 109
Christophe Valat, Benoit Limoges, Denis Huet and Jean-Louis Romette
Abstract:
Low-cost disposable immunosensors were produced by covalent binding of Protein A or G on graphite-polystyrene screen-printed electrodes, and they were used in a fully automated flow injection analysis (FIA) system, allowing the kinetics of IgG binding to Protein A or G to be improved by forced convection. The displacement of rabbit IgG bound to Protein A or G by mouse IgG isotypes (IgG(1) or IgG(2a)) was studied. A FIA immunoassay of mouse IgG(2a) was performed at a Protein A-based immunosensor with a good sensibility (down to 0.02 µg ml-1) and a total assay time of 19 min. It was shown that the immunosensor combines the advantages of being reusable for more than 30 assay cycles in flow injection analysis, and disposable when necessary.
Immunoglobulin G, mouse
Apparatus
Immobilized protein
