Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Sensor
Classification:
Sensor
-> surface plasmon resonance
Citations 3
"Determination Of Simazine In Water Samples By Waveguide Surface Plasmon Resonance"
Anal. Chim. Acta
1997 Volume 338, Issue 1-2 Pages 109-117
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The biosensor was incorporated into a FIA system and PBS of pH 7.4 was used as the carrier stream. The assays were carried out by incubating the buffered sample with simazine antibodies for 5 min. A portion (100 µL) of the solution was injected into the FIA system and the carrier stream flow was stopped for 8 min when the sample plug reacted the detector cell to allow the free antibodies to react with the immobilized triazine derivative. After recording the signal the biosensor was regenerated by pumping 2 g/l pepsin in PBS of pH 1.9 and 1% propionic acid in aqueous 50% acetonitrile. The system was calibrated for up to 100 µg/l simazine, the detection limit was 0.2 µg/l and the day-to-day RSD (n = 5) were The cross reactivities of atrazine and terbutylazine were 61% and 62%, respectively. The method was applied to surface and ground water samples and the results were confirmed by chromatographic analysis. 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Biochem.', 'journal_id' => '0570', 'fadid' => 'ANBI1995V0226P00175', 'year' => '1995', 'volume' => '226', 'issue' => '1', 'startpage' => '175', 'endpage' => '181', 'type' => 'Journal Article', 'analytes' => ';2226;', 'matrices' => ';0007;', 'techniques' => ';0440;0431;', 'keywords' => '', 'abstract' => 'The use of antibiotics and chemotherapeutics in animal husbandry has led to the occurrence of veterinary drug residues in all types of food of animal origin. Due to the specification of toxicologically based maximum residue levels for a large number of substances, existing control strategies need even faster and more sensitive methods to meet new and more rigorous regulations. The applicability of an immunosensor device for biospecific interaction analysis was investigated and the development of an assay for analysis of sulfamethazine (SMZ) in milk is described. SMZ was covalently immobilized to a carboxymethyldextran-modified gold film. 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The results were not influenced in such a way that the risk for so-called false-positive findings was obvious.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '2', 'urlcheck' => '2014-10-12 09:15:59', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => null, 'pauthor' => 'NA', 'address' => 'Swedish Univ. Agric. Sci., Dept. Food Sci., Uppsala 750 07 Sweden', 'email' => 'NA', 'notes' => null, 'url' => '10.1006/abio.1995.1206', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay', Anal. 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Ser.', 'journal_id' => '1346', 'fadid' => 'NASS1997V0037P00321', 'year' => '1997', 'volume' => '37', 'issue' => '1', 'startpage' => '321', 'endpage' => '322', 'type' => 'Journal Article', 'analytes' => ';0847;', 'matrices' => '', 'techniques' => ';0440;', 'keywords' => '', 'abstract' => 'In order to realize immediate detection of a double stranded DNA amplified by Polymerase Chain Reaction (PCR), we applied Peptide Nucleic Acid (PNA) to the probe of DNA detection system using Surface Plasmon Resonance (SPR). 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Acta', 'journal_id' => '0584', 'fadid' => 'ANCA1997V0338P00109', 'year' => '1997', 'volume' => '338', 'issue' => '1-2', 'startpage' => '109', 'endpage' => '117', 'type' => 'Journal Article', 'analytes' => ';2166;', 'matrices' => ';0371;', 'techniques' => ';0431;0440;', 'keywords' => ';0217;0446;0208;', 'abstract' => 'A waveguide surface plasmon resonance biosensor, equipped with low-index glass waveguides and a 10 mW He-Ne laser (632.8 nm), was evaluated for determining simazine in natural waters. The interaction length (3 mm) of the biosensor was coated with a Au film (53±3 mm thickness) and a triazine derivative, 4-chloro-6-(isopropylamino)-1,3,5-triazine-2-(6'-amino)caproic acid. The biosensor was incorporated into a FIA system and PBS of pH 7.4 was used as the carrier stream. The assays were carried out by incubating the buffered sample with simazine antibodies for 5 min. 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Abstract:
A waveguide surface plasmon resonance biosensor, equipped with low-index glass waveguides and a 10 mW He-Ne laser (632.8 nm), was evaluated for determining simazine in natural waters. The interaction length (3 mm) of the biosensor was coated with a Au film (53±3 mm thickness) and a triazine derivative, 4-chloro-6-(isopropylamino)-1,3,5-triazine-2-(6'-amino)caproic acid. The biosensor was incorporated into a FIA system and PBS of pH 7.4 was used as the carrier stream. The assays were carried out by incubating the buffered sample with simazine antibodies for 5 min. A portion (100 µL) of the solution was injected into the FIA system and the carrier stream flow was stopped for 8 min when the sample plug reacted the detector cell to allow the free antibodies to react with the immobilized triazine derivative. After recording the signal the biosensor was regenerated by pumping 2 g/l pepsin in PBS of pH 1.9 and 1% propionic acid in aqueous 50% acetonitrile. The system was calibrated for up to 100 µg/l simazine, the detection limit was 0.2 µg/l and the day-to-day RSD (n = 5) were The cross reactivities of atrazine and terbutylazine were 61% and 62%, respectively. The method was applied to surface and ground water samples and the results were confirmed by chromatographic analysis. The method was not suitable for analyzing soil water samples due to the strong non-selective polyanion-polycation binding to the transducer surface.
Simazine
Water
Interferences
Stopped-flow
Immobilized reagent
"Determination Of Sulfamethazine Residues In Milk By A Surface Plasmon Resonance-based Biosensor Assay"
Anal. Biochem.
1995 Volume 226, Issue 1 Pages 175-181
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The biosensor was incorporated into a FIA system and PBS of pH 7.4 was used as the carrier stream. The assays were carried out by incubating the buffered sample with simazine antibodies for 5 min. A portion (100 µL) of the solution was injected into the FIA system and the carrier stream flow was stopped for 8 min when the sample plug reacted the detector cell to allow the free antibodies to react with the immobilized triazine derivative. After recording the signal the biosensor was regenerated by pumping 2 g/l pepsin in PBS of pH 1.9 and 1% propionic acid in aqueous 50% acetonitrile. The system was calibrated for up to 100 µg/l simazine, the detection limit was 0.2 µg/l and the day-to-day RSD (n = 5) were The cross reactivities of atrazine and terbutylazine were 61% and 62%, respectively. The method was applied to surface and ground water samples and the results were confirmed by chromatographic analysis. 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Spiked samples with known concentrations of SMZ were prepared in HBS buffer and skim and raw milk for construction of standard curves. Polyclonal antibodies against SMZ were added to the sample and the immobilized surface was used to determine the amount of free antibodies by surface plasmon resonance detection. After each measurement the surface was regenerated by NaOH and HCl. In milk, the mean relative standard deviation of the assay was approximately 2% and the limit of detection less than 1 ppb. By introduction of a secondary sheep anti-rabbit antibody, the use of specific antibody could be reduced. Milk samples from the individual cow, herd, and tanker levels were analyzed and the relative standard deviations within each sample category were 4.4, 2.4, and 2.2%, respectively. The effect of some potential interferences, e.g., high somatic cells, bacterial contamination, and preservatives, was investigated. 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Abstract:
The use of antibiotics and chemotherapeutics in animal husbandry has led to the occurrence of veterinary drug residues in all types of food of animal origin. Due to the specification of toxicologically based maximum residue levels for a large number of substances, existing control strategies need even faster and more sensitive methods to meet new and more rigorous regulations. The applicability of an immunosensor device for biospecific interaction analysis was investigated and the development of an assay for analysis of sulfamethazine (SMZ) in milk is described. SMZ was covalently immobilized to a carboxymethyldextran-modified gold film. Spiked samples with known concentrations of SMZ were prepared in HBS buffer and skim and raw milk for construction of standard curves. Polyclonal antibodies against SMZ were added to the sample and the immobilized surface was used to determine the amount of free antibodies by surface plasmon resonance detection. After each measurement the surface was regenerated by NaOH and HCl. In milk, the mean relative standard deviation of the assay was approximately 2% and the limit of detection less than 1 ppb. By introduction of a secondary sheep anti-rabbit antibody, the use of specific antibody could be reduced. Milk samples from the individual cow, herd, and tanker levels were analyzed and the relative standard deviations within each sample category were 4.4, 2.4, and 2.2%, respectively. The effect of some potential interferences, e.g., high somatic cells, bacterial contamination, and preservatives, was investigated. The results were not influenced in such a way that the risk for so-called false-positive findings was obvious.
Sulfamethazine
Milk
"Novel DNA Detection System Of Flow Injection Analysis. 2. The Distinctive Properties Of A Novel System Employing PNA (peptide Nucleic Acid) As A Probe For Specific DNA Detection"
Nucleic Acids Symp. Ser.
1997 Volume 37, Issue 1 Pages 321-322
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The assays were carried out by incubating the buffered sample with simazine antibodies for 5 min. A portion (100 µL) of the solution was injected into the FIA system and the carrier stream flow was stopped for 8 min when the sample plug reacted the detector cell to allow the free antibodies to react with the immobilized triazine derivative. After recording the signal the biosensor was regenerated by pumping 2 g/l pepsin in PBS of pH 1.9 and 1% propionic acid in aqueous 50% acetonitrile. The system was calibrated for up to 100 µg/l simazine, the detection limit was 0.2 µg/l and the day-to-day RSD (n = 5) were The cross reactivities of atrazine and terbutylazine were 61% and 62%, respectively. The method was applied to surface and ground water samples and the results were confirmed by chromatographic analysis. The method was not suitable for analyzing soil water samples due to the strong non-selective polyanion-polycation binding to the transducer surface.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '9', 'urlcheck' => '2014-10-11 16:22:19', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '01174', 'pauthor' => '!Mouvet, C.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1016/S0003-2670(96)00443-6', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Determination of simazine in water samples by waveguide surface plasmon resonance', Anal. Chim. Acta, 1997 338(1-2) 109-117', 'firstchar' => 'D', 'twochars' => 'De', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array( [maximum depth reached] ), 'Keyword' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '009523', 'authors' => 'Sternesjo, A.;Mellgren, C.;Bjorck, L.', 'authorsweb' => 'Sternesjo A., Mellgren C. and Bjorck L.', 'title' => 'Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay', 'journal' => 'Anal. Biochem.', 'journal_id' => '0570', 'fadid' => 'ANBI1995V0226P00175', 'year' => '1995', 'volume' => '226', 'issue' => '1', 'startpage' => '175', 'endpage' => '181', 'type' => 'Journal Article', 'analytes' => ';2226;', 'matrices' => ';0007;', 'techniques' => ';0440;0431;', 'keywords' => '', 'abstract' => 'The use of antibiotics and chemotherapeutics in animal husbandry has led to the occurrence of veterinary drug residues in all types of food of animal origin. Due to the specification of toxicologically based maximum residue levels for a large number of substances, existing control strategies need even faster and more sensitive methods to meet new and more rigorous regulations. The applicability of an immunosensor device for biospecific interaction analysis was investigated and the development of an assay for analysis of sulfamethazine (SMZ) in milk is described. SMZ was covalently immobilized to a carboxymethyldextran-modified gold film. Spiked samples with known concentrations of SMZ were prepared in HBS buffer and skim and raw milk for construction of standard curves. Polyclonal antibodies against SMZ were added to the sample and the immobilized surface was used to determine the amount of free antibodies by surface plasmon resonance detection. After each measurement the surface was regenerated by NaOH and HCl. In milk, the mean relative standard deviation of the assay was approximately 2% and the limit of detection less than 1 ppb. By introduction of a secondary sheep anti-rabbit antibody, the use of specific antibody could be reduced. Milk samples from the individual cow, herd, and tanker levels were analyzed and the relative standard deviations within each sample category were 4.4, 2.4, and 2.2%, respectively. The effect of some potential interferences, e.g., high somatic cells, bacterial contamination, and preservatives, was investigated. The results were not influenced in such a way that the risk for so-called false-positive findings was obvious.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '2', 'urlcheck' => '2014-10-12 09:15:59', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => null, 'pauthor' => 'NA', 'address' => 'Swedish Univ. Agric. Sci., Dept. Food Sci., Uppsala 750 07 Sweden', 'email' => 'NA', 'notes' => null, 'url' => '10.1006/abio.1995.1206', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Determination of sulfamethazine residues in milk by a surface plasmon resonance-based biosensor assay', Anal. Biochem., 1995 226(1) 175-181', 'firstchar' => 'D', 'twochars' => 'De', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array( [maximum depth reached] ), 'Keyword' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '014096', 'authors' => 'Kai, E.;Sawata, S.;Ikebukuro, K.;Iida, T.;Honda, T.;Karube, I.', 'authorsweb' => 'Kai E, Sawata S, Ikebukuro K, Iida T, Honda T, Karube I.', 'title' => 'Novel DNA detection system of flow injection analysis. 2. The distinctive properties of a novel system employing PNA (peptide nucleic acid) as a probe for specific DNA detection', 'journal' => 'Nucleic Acids Symp. Ser.', 'journal_id' => '1346', 'fadid' => 'NASS1997V0037P00321', 'year' => '1997', 'volume' => '37', 'issue' => '1', 'startpage' => '321', 'endpage' => '322', 'type' => 'Journal Article', 'analytes' => ';0847;', 'matrices' => '', 'techniques' => ';0440;', 'keywords' => '', 'abstract' => 'In order to realize immediate detection of a double stranded DNA amplified by Polymerase Chain Reaction (PCR), we applied Peptide Nucleic Acid (PNA) to the probe of DNA detection system using Surface Plasmon Resonance (SPR). We report our success in immediate detection of PCR products solution with high sequence-specificity.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'No', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '0', 'urlcheck' => '2014-10-13 19:09:08', 'urlcheckcode' => 'HTTP/1.1 200 OK', 'pauthor_id' => '00227', 'pauthor' => '!Karube, I.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => 'www.ncbi.nlm.nih.gov/pubmed/9586129', 'urltype' => 'absurl', 'gotpdf' => 'no', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Novel DNA detection system of flow injection analysis. 2. The distinctive properties of a novel system employing PNA (peptide nucleic acid) as a probe for specific DNA detection', Nucleic Acids Symp. 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Ser.', 'journal_id' => '1346', 'fadid' => 'NASS1997V0037P00321', 'year' => '1997', 'volume' => '37', 'issue' => '1', 'startpage' => '321', 'endpage' => '322', 'type' => 'Journal Article', 'analytes' => ';0847;', 'matrices' => '', 'techniques' => ';0440;', 'keywords' => '', 'abstract' => 'In order to realize immediate detection of a double stranded DNA amplified by Polymerase Chain Reaction (PCR), we applied Peptide Nucleic Acid (PNA) to the probe of DNA detection system using Surface Plasmon Resonance (SPR). We report our success in immediate detection of PCR products solution with high sequence-specificity.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'No', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '0', 'urlcheck' => '2014-10-13 19:09:08', 'urlcheckcode' => 'HTTP/1.1 200 OK', 'pauthor_id' => '00227', 'pauthor' => '!Karube, I.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => 'www.ncbi.nlm.nih.gov/pubmed/9586129', 'urltype' => 'absurl', 'gotpdf' => 'no', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Novel DNA detection system of flow injection analysis. 2. The distinctive properties of a novel system employing PNA (peptide nucleic acid) as a probe for specific DNA detection', Nucleic Acids Symp. Ser., 1997 37(1) 321-322', 'firstchar' => 'N', 'twochars' => 'No', 'CitationsTechnique' => array( 'id' => '014743', 'citation_id' => '014096', 'technique_id' => '0440' ), 'Analyte' => array( (int) 0 => array( 'id' => '00847', 'name' => 'DNA', 'iupac_name' => '1,4-dihydroxynaphthalene-2-carboxylic acid', 'casrn' => 'NA', 'synonyms' => 'Deoxyribonucleic acid', 'total' => '10', 'inchi' => 'InChI=1S/C11H8O4/c12-9-5-8(11(14)15)10(13)7-4-2-1-3-6(7)9/h1-5,12-13H,(H,14,15)', 'inchikey' => 'VOJUXHHACRXLTD-UHFFFAOYSA-N', 'formula' => 'C11H8O4', 'oxstate' => 'Zero', 'url' => '', 'charge' => '0', 'class1' => 'Biological', 'class2' => 'NA', 'class3' => 'NA', 'class4' => 'Molecule', 'class5' => 'Acids', 'isgroup' => 'no', 'checked' => 'yes', 'citation_count' => '0', 'updated' => '2015-10-23 09:57:06', 'first' => 'D', 'nametotal' => 'DNA**10', 'AnalytesCitation' => array( [maximum depth reached] ) ) ), 'Matrix' => array(), 'Keyword' => array() ) $i = (int) 2 $path = '' $a = '' $url = 'http://www.ncbi.nlm.nih.gov/pubmed/9586129' $aus = 'Kai E, Sawata S, Ikebukuro K, Iida T, Honda T, Karube I.'include - APP/View/Elements/citation.ctp, line 40 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Techniques/view.ctp, line 52 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 968 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 109
Abstract:
In order to realize immediate detection of a double stranded DNA amplified by Polymerase Chain Reaction (PCR), we applied Peptide Nucleic Acid (PNA) to the probe of DNA detection system using Surface Plasmon Resonance (SPR). We report our success in immediate detection of PCR products solution with high sequence-specificity.
DNA