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"Planar Waveguide Immunosensor With Fluorescent Liposome Amplification"
Anal. Chem. 1992 Volume 64, Issue 1 Pages 55-60

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	'techniques' => ';0437;0431;0252;',
	'keywords' => ';0245;',
	'abstract' => 'A flow injection analysis (FIA) manifold (described) with an anti-theophylline derivatized planar waveguide optical sensor was developed. The planar waveguides were produced by immersing Corning 0211 cover slips into AgNO3 - NaNO3 (1:9) at 280&deg;C for 15 min to 1 h, and monoclonal mouse anti-theophylline IgG was coupled to the waveguide surface. Samples of theophylline (50 &micro;L) were injected into the FIA manifold, and the flow was stopped for 5 min to 1 h to allow equilibration of the sample to the sensor surface. After incubation, the flow cell was flushed, followed by detection of the specifically bound liposomes at 516 nm (excitation at 488 nm). Antibody activity was regenerated by adding 1-O-octyl &beta;-D-glucopyranoside for 2 min, then rinsing for 20 min with phosphate-buffered saline solution (pH 7.4). Sensors capable of >15 sequential measurements demonstrated >10% precision.',
	'language' => 'English',
	'updated' => '2020-12-28 11:25:15',
	'sjccheck' => 'Yes',
	'sjccheckdate' => '0000-00-00 00:00:00',
	'hits' => '1',
	'urlcheck' => '2014-10-11 18:36:41',
	'urlcheckcode' => 'HTTP/1.1 302 Found',
	'pauthor_id' => null,
	'pauthor' => 'Choquette, S.J.',
	'address' => 'National Institute of Standards and Technology, Gaithersburg, Maryland 20899',
	'email' => 'NA',
	'notes' => null,
	'url' => '10.1021/ac00025a011',
	'urltype' => 'doi',
	'gotpdf' => 'yes',
	'partial' => 'no',
	'notanalyte' => '',
	'citation' => ''Planar waveguide immunosensor with fluorescent liposome amplification', Anal. Chem., 1992 64(1) 55-60',
	'firstchar' => 'P',
	'twochars' => 'Pl',
	'CitationsTechnique' => array(
		'id' => '006467',
		'citation_id' => '008652',
		'technique_id' => '0437'
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	'Analyte' => array(
		(int) 0 => array(
			'id' => '02312',
			'name' => 'Theophylline',
			'iupac_name' => '1,3-dimethyl-7H-purine-2,6-dione',
			'casrn' => '5967-84-0',
			'synonyms' => 'Theophylline monohydrate',
			'total' => '26',
			'inchi' => 'InChI=1S/C7H8N4O2/c1-10-5-4(8-3-9-5)6(12)11(2)7(10)13/h3H,1-2H3,(H,8,9)',
			'inchikey' => 'ZFXYFBGIUFBOJW-UHFFFAOYSA-N',
			'formula' => 'C7H8N4O2',
			'oxstate' => 'Zero',
			'url' => '',
			'charge' => '0',
			'class1' => 'Organic compound',
			'class2' => 'NA',
			'class3' => 'NA',
			'class4' => 'Molecule',
			'class5' => 'Drugs',
			'isgroup' => '',
			'checked' => 'yes',
			'citation_count' => '0',
			'updated' => '2015-10-23 21:38:09',
			'first' => 'T',
			'nametotal' => 'Theophylline**26',
			'AnalytesCitation' => array(
				[maximum depth reached]
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		)
	),
	'Matrix' => array(),
	'Keyword' => array(
		(int) 0 => array(
			'id' => '0245',
			'type' => 'Reagent',
			'keyword' => 'Liposomes',
			'newKeyword' => '',
			'synonyms' => '',
			'fao' => '',
			'total' => '21',
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			'keytotal' => 'Liposomes**21',
			'CitationsKeyword' => array(
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)
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$path = ''
$a = ''
$url = 'http://dx.doi.org/10.1021/ac00025a011'
$aus = 'Steven J. Choquette, Laurie Locascio-Brown, and Richard A. Durst'
include - APP/View/Elements/citation.ctp, line 40
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::_renderElement() - CORE/Cake/View/View.php, line 1224
View::element() - CORE/Cake/View/View.php, line 418
include - APP/View/Techniques/view.ctp, line 52
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 968
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 109