FAD: Techniques

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

Sensor

Classification: Sensor -> bilayer lipid membrane

Citations 1

'Bilayer Lipid Membrane As A Generic Electrochemical Transducer Of Hydrolytic Enzyme Reactions', Biosens. Bioelectron., 1994 9(3) 179-188

"Bilayer Lipid Membrane As A Generic Electrochemical Transducer Of Hydrolytic Enzyme Reactions"
Biosens. Bioelectron. 1994 Volume 9, Issue 3 Pages 179-188

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Nikolelis, D.P.;Tzanelis, M.G.;Krull, U.J.

Abstract: Bilayer lipid membranes (BLM) were used as generic transducers to monitor hydrolytic enzyme reactions at the membrane surface. The reactions of urease and penicillase with urea and penicillin were used as test reactions. The BLM could be used as generic transducers of pH; the increase in pH being dependent on the quantity and extent of dipalmitoylphosphatidic acid up to 35%. The results were consistent with an electrostatic mechanism of perturbation of the BLM surface structure. Detection limits were 40 µM-urea and 0.1 mM penicillin. The planar membranes were stable for a few hours and would be best suited to stopped-flow or flow injection systems where solution movement can be controlled.

Urea Penicillin Biological Stopped-flow Bilayer lipid membranes