FAD: Techniques

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Microscopy

Classification: Microscopy -> molecular absorption -> infrared -> fourier transform
Citations 1

'FTIR Microscopy As Novel Detection System In Micro Flow Injection Analysis', Anal. Methods Instrum., 1995 2(1) 52-54

"FTIR Microscopy As Novel Detection System In Micro Flow Injection Analysis"
Anal. Methods Instrum. 1995 Volume 2, Issue 1 Pages 52-54
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Kellner, Robert; Lendl, Bernhard
Abstract: A Bruker 113v FTIR spectrometer was coupled with a Bruker IR microscope equipped with a HgCdTe detector. A flow-through cell was developed for this system comprising three parts; the central part of which was constructed of PVC with an inner volume of 1.6 µL and an optical pathlength of 500 µm. The windows consisted of two Si plates (diameter 5.5 mm, thickness 0.75 mm). As the IR beam is focused by the FTIR microscope down to 80 µm it was possible to probe volumes of 10 nl. IBMK was determined in hexane in the ppm range as a model substance. Possible future applications of the system as a µtotal analysis system are discussed.
Methyl isobutyl ketone Organic compound Apparatus MIBK