"Continuous-flow Matrix-assisted Laser Desorption Ionization Mass Spectrometry"
Anal. Chem.
1993 Volume 65, Issue 4 Pages 493-495
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Liang Li, Alan P. L. Wang, and Larry D. Coulson
Abstract:
A 1-m silica tube (75 µm i.d., 363 µm o.d.) is inserted into a stainless steel tube (6.35 mm i.d., 1.27 cm o.d.) and extends to the probe tip (5 mm long, 5 mm o.d., 1 mm i.d.) made from Vespel; the top section (~ 7.62 cm) of the silica is coated with Vespel as insulation, and a stainless steel frit is fitted into the end of the tip. The probe is inserted via a solid-probe lock into a time-of-flight mass spectrometer, in which ions are detected by using a multi-channel plate detector. A solution of peptide or protein in aqueous 0.1% trifluoroacetic acid is mixed (1:1:1:1) with methanol, ethanediol and 3-nitrobenzyl alcohol and is introduced continuously (1 to 5 µL min-1) by a micro-syringe pump onto the tip of the flow probe; a flow injection variant is also described. A Nd:YAG laser operating at 266 nm and repetition rate 10 Hz is used to effect desorption ionization; the conditions used for MS are given. The mass spectra of myoglobin obtained by this technique and with a solid probe are similar, but the flow system can provide a stable signal for extended periods; however, the signal in the dynamic mode is less intense by a factor of 5 to 10. Use of the flow injection variant with bacitracin affords reproducible signals with an acceptable degree of peak tailing and no memory effect. Mass resolution is ~10 for the compounds examined but is higher for molecules of mol. wt. 1000.
Peptides
Proteins
Laser
