"Amperometric Determination Of Lactic Acid. Applications On Milk Samples"
Anal. Lett.
1988 Volume 21, Issue 5 Pages 727-740
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Pilloton, R.;Nwosu, T.N.;Mascini, M.
Abstract:
A H2O2-selective electrode (Instrumentation Lab., Milan) was coated with L-lactate oxidase as active ingredient, immobilized on a nylon net and protected by a cellulose acetate dialysis membrane, and was applied in the determination of lactate in milk in a flow injection analysis system in which the flow rate was 0.2 mL min-1, the detector cell was of ~40 µL dead volume and amperometric detection was at +0.65 V vs. a Ag - AgCl reference electrode. The carrier stream was 0.1 M phosphate buffer at pH 7.0. The calibration graph was rectilinear up to 75 µM-lactate.
Lactic acid
Milk
Cellulose acetate
Membrane
Immobilized enzyme
Dialysis