"Detection Of Nitric Oxide Using An Optical Sensor"
Indian J. Chem. Technol.
1998 Volume 5, Issue 6 Pages 402-404
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PANDEY P. C.
Abstract:
An optical sensor for nitric oxide (NO) is described. The sensor involves a flow injection analysis (FIA) system coupled with an evanescent wave (EW) sensor employing total internal reflection of fluorescence radiation (TIRF). The detection is based on the measurement of the decrease in fluorescence response in the presence of NO of a highly fluorescent product which is the complex of glutathione and 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM). CPM forms a highly fluorescent product with thiol containing compound i.e. cysteine/glutathione which can be detected using an evanescent fluorosensor. NO form a stable complex with glutathione/cysteine which then does not react with CPM to form fluorescent product. Thus there is a decrease in the fluorescence signal on increasing concentration. of NO with constant concentration. of glutathione. An excellent FIA response was observed on the injection of the constant concentration. of CPM-glutathione product obtained by treating constant concentration. of glutathione with varying concentration. of NO. The lowest detection limit of NO is found to the order of 0.10 µM.
Nitric oxide
Indirect