Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Electrode
Citations 2
"Xanthine And Hypoxanthine Sensors Based On Xanthine Oxidase Immobilized On A CuPtCl6 Chemically Modified Electrode And Liquid Chromatography Electrochemical Detection"
Anal. Chim. Acta
2000 Volume 414, Issue 1-2 Pages 205-213
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Abstract:
Xanthine oxidase has been immobilized on the surface of a CuPtCl6/glassy carbon chemically modified electrode. The enzyme electrode displays a quick and sensitive response to xanthine and hypoxanthine. The observed steady-state current is due to the oxidation of hydrogen peroxide Liberated during the enzyme reaction on the surface of the enzyme electrode. The effects of enzyme loading, pH, temperature, and applied potential have been discussed. The enzyme electrode was characterized as an amperometric sensor for xanthine (6 x 10^-7-2 x 10^-4 M) and hypoxanthine (5 x 10^-7-2 x 10^-4 M) with the detection limit of 1 x 10^-7 M. Liquid chromatographic separation coupled with the enzyme electrode as an electrochemical detector was used to analyze the mixtures of xanthine and hypoxanthine.
Xanthine
Hypoxanthine
Detector
Optimization
"Constant-potential Amperometric Detection Of Carbohydrates At A Copper-based Chemically Modified Electrode"
Anal. Chem.
1989 Volume 61, Issue 8 Pages 852-856
Notice (8): Undefined variable: uid [APP/View/Elements/citation.ctp, line 40]Sunil V. Prabhu and Richard P. BaldwinCode Context?><?phpif($uid!='') {$viewFile = '/home/stuchalk/public_html/fad/app/View/Elements/citation.ctp' $dataForView = array( 'data' => array( 'Technique' => array( 'id' => '0228', 'label' => 'Electrode', 'level1' => 'Electrode', 'level2' => 'vitreous carbon', 'level3' => 'copper coated', 'level4' => '', 'level5' => '', 'synonyms' => '', 'champ' => '', 'total' => '2', 'updated' => '0000-00-00 00:00:00', 'name' => 'Electrode, vitreous carbon, copper coated', 'nametotal' => 'Electrode, vitreous carbon, copper coated**2', 'first' => 'E' ), 'Citation' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'c' => array( 'id' => '008538', 'authors' => 'Prabhu, S.V.;Baldwin, R.P.', 'authorsweb' => 'Sunil V. Prabhu and Richard P. 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Chem.', 'journal_id' => '0499', 'fadid' => 'ANCH1989V0061P00852', 'year' => '1989', 'volume' => '61', 'issue' => '8', 'startpage' => '852', 'endpage' => '856', 'type' => 'Journal Article', 'analytes' => ';0508;', 'matrices' => '', 'techniques' => ';0228;0001;', 'keywords' => '', 'abstract' => 'A chemically modified electrode was prepared by coating a vitreous-carbon electrode with a Cu(II) layer, which decreased the overpotential for carbohydrate oxidation compared to that at unmodified carbon electrodes. The electrode was used for constant potential amperometric detection of reducing and non-reducing sugars separated by HPLC on a column (15 cm x 4 mm) of Dionex HPIC-AS6. The Cu layer catalyzed the sugar oxidation in basic solution when a potential sufficiently positive to generate Cu(III) was applied. In flow injection analysis and HPLC, electrode response was stable for >5 h (signal loss 10%). 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Baldwin'include - APP/View/Elements/citation.ctp, line 40 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Techniques/view.ctp, line 52 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 968 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 109
Abstract:
A chemically modified electrode was prepared by coating a vitreous-carbon electrode with a Cu(II) layer, which decreased the overpotential for carbohydrate oxidation compared to that at unmodified carbon electrodes. The electrode was used for constant potential amperometric detection of reducing and non-reducing sugars separated by HPLC on a column (15 cm x 4 mm) of Dionex HPIC-AS6. The Cu layer catalyzed the sugar oxidation in basic solution when a potential sufficiently positive to generate Cu(III) was applied. In flow injection analysis and HPLC, electrode response was stable for >5 h (signal loss 10%). With anion-exchange chromatography in 0.15 M NaOH, mono- and disaccharides were separated and detected in sub ng amounts at an applied potential of +0.48 V vs. Ag - AgCl.
Carbohydrates