University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Citations 1

"High-throughput Flow Injection Analysis Of Glucose And Glutamate In Food And Biological Samples By Using Enzyme/polyion Complex-bilayer Membrane-based Electrodes As The Detectors"
Biosens. Bioelectron. 1998 Volume 13, Issue 7-8 Pages 809-815

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Fumio Mizutani*, Yukari Sato, Yoshiki Hirata and Soichi Yabuki

Abstract: The concentration of glucose was determined by a combination of flow injection analysis (FIA) with amperometric enzyme sensor detection. The enzyme sensor was prepared by immobilizing glucose oxidase on an electrode coated with a polyion complex layer consisting of poly-L-lysine and poly(4-styrenesulfonate). The inner, polyion complex layer was useful for preventing electrochemical interferents (e.g., L-ascorbic acid, uric acid and acetaminophen) from reaching the electrode surface, which was effective for reducing the interferential responses upon the injections of biological and food samples. The sensor-based system could be used for the determination of glucose from 10 µM to 3 mM with the sampling rate of 180 h-1, and was stable for more than 2 mo. An FIA system for determining L-glutamic acid (3 µM-0.5 mM) was also prepared by using an enzyme electrode based on a glutamate oxidase/polyion complex-bilayer as the detector.
Glucose l-Glutamic acid Food Biological Interferences Apparatus Detector