"Microporous Gold Electrodes As Combined Biosensor/electrochemical Detectors In Flowing Streams"
Electroanalysis
1998 Volume 10, Issue 3 Pages 157-162
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Michael W. Ducey Jr., Mark E. Meyerhoff
Abstract:
Microporous gold electrodes are described for use as flow-through biosensor/electrochemical detectors in both flow injection analysis (FIA) as well as post-column detection in HPLC. Electrodes prepared by the covalent immobilization of tyrosinase onto the electrode surface (via a chemisorbed layer of thioctic acid and polylysine) are used to demonstrate the utility of the electrode. Such electrodes are shown to respond to a variety of substituted phenols under FIA conditions at applied potential of only -50 mV (vs. Ag/AgCl). Detection limits of 0.5 µM catechol and 3.4 µM phenol were achieved with a sample throughput rate of 38 samples/h. The electrode is shown to be stable for up to 14 days of use. The porous bioelectrode is shown to be useful as a post-column detector for substituted phenols following their separation by HPLC. Various techniques for immobilization of the enzyme onto the electrode surface (covalent and noncovalent) as well as factors affecting sensitivity and through-put (buffer ionic strength and addition of organic modifier) are discussed.
Apparatus
Detector
Optimization
