Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Electrode
Citations 2
"Biamperometric Determination Of Glycerol And Triglycerides With Open Tubular Carbon Electrodes In Flow Streams"
Anal. Chim. Acta
1979 Volume 106, Issue 2 Pages 225-231
Notice (8): Undefined variable: uid [APP/View/Elements/citation.ctp, line 40]Abdulrahman S. Attiyat and Gary D. ChristianCode Context?><?phpif($uid!='') {$viewFile = '/home/stuchalk/public_html/fad/app/View/Elements/citation.ctp' $dataForView = array( 'data' => array( 'Technique' => array( 'id' => '0072', 'label' => 'Electrode', 'level1' => 'Electrode', 'level2' => 'carbon', 'level3' => 'open tubular', 'level4' => '', 'level5' => '', 'synonyms' => '', 'champ' => '', 'total' => '2', 'updated' => '0000-00-00 00:00:00', 'name' => 'Electrode, carbon, open tubular', 'nametotal' => 'Electrode, carbon, open tubular**2', 'first' => 'E' ), 'Citation' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'c' => array( 'id' => '003879', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'Abdulrahman S. Attiyat and Gary D. Christian', 'title' => 'Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', 'journal' => 'Anal. Chim. Acta', 'journal_id' => '0584', 'fadid' => 'ANCA1979V0106P00225', 'year' => '1979', 'volume' => '106', 'issue' => '2', 'startpage' => '225', 'endpage' => '231', 'type' => 'Journal Article', 'analytes' => ';1103;2414;', 'matrices' => '', 'techniques' => ';0013;0046;0072;', 'keywords' => ';0147;', 'abstract' => 'Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorase-catalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 x 10^-5 - 6 x 10^-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '6', 'urlcheck' => '2014-10-11 15:40:48', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1016/S0003-2670(01)85005-4', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', Anal. Chim. Acta, 1979 106(2) 225-231', 'firstchar' => 'B', 'twochars' => 'Bi', 'CitationsTechnique' => array( 'id' => '000561', 'citation_id' => '003879', 'technique_id' => '0072' ), 'Analyte' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Matrix' => array(), 'Keyword' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'i' => (int) 0 ) $data = array( 'Technique' => array( 'id' => '0072', 'label' => 'Electrode', 'level1' => 'Electrode', 'level2' => 'carbon', 'level3' => 'open tubular', 'level4' => '', 'level5' => '', 'synonyms' => '', 'champ' => '', 'total' => '2', 'updated' => '0000-00-00 00:00:00', 'name' => 'Electrode, carbon, open tubular', 'nametotal' => 'Electrode, carbon, open tubular**2', 'first' => 'E' ), 'Citation' => array( (int) 0 => array( 'id' => '003879', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'Abdulrahman S. Attiyat and Gary D. Christian', 'title' => 'Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', 'journal' => 'Anal. Chim. Acta', 'journal_id' => '0584', 'fadid' => 'ANCA1979V0106P00225', 'year' => '1979', 'volume' => '106', 'issue' => '2', 'startpage' => '225', 'endpage' => '231', 'type' => 'Journal Article', 'analytes' => ';1103;2414;', 'matrices' => '', 'techniques' => ';0013;0046;0072;', 'keywords' => ';0147;', 'abstract' => 'Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorase-catalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 x 10^-5 - 6 x 10^-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '6', 'urlcheck' => '2014-10-11 15:40:48', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1016/S0003-2670(01)85005-4', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', Anal. Chim. Acta, 1979 106(2) 225-231', 'firstchar' => 'B', 'twochars' => 'Bi', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array([maximum depth reached]), 'Keyword' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '009580', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'NA', 'title' => 'Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', 'journal' => 'Anal. Lett.', 'journal_id' => '0820', 'fadid' => 'ANLE1987V0020P01099', 'year' => '1987', 'volume' => '20', 'issue' => '7', 'startpage' => '1099', 'endpage' => '1113', 'type' => 'Journal Article', 'analytes' => ';0931;', 'matrices' => ';0131;', 'techniques' => ';0013;0072;', 'keywords' => ';0258;', 'abstract' => 'A three-channel flow system was used, the first channel pumping NAD+ and dihydrolipoamide dehydrogenase(I), the second alcohol dehydrogenase(II) and K3Fe(CN)6 and the third diluted blood samples, all at the same flow rate. The three channels merged at a single point, thence passing through a flow cell containing two open-tubular carbon electrodes, with a potential difference of 200 mV. In the presence of ethanol and II, NAD+ was reduced to NADH; this was oxidized back to NAD+ by Fe(CN)63- in the presence of I. The Fe(CN)64- formed was detected at the electrodes. Blood samples were initially diluted 100-fold with 0.1 M Tris buffer (pH 8.5). The detection limit was 0.15 µg mL-1 of ethanol in blood and coefficient of variation were 4%. On eleven samples, correlation with a GC method was good (r = 0.98).', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '2007-03-29 17:18:00', 'hits' => '3', 'urlcheck' => '2014-10-12 00:06:26', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1080/00032718708064594', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', Anal. Lett., 1987 20(7) 1099-1113', 'firstchar' => 'C', 'twochars' => 'Co', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array( [maximum depth reached] ), 'Keyword' => array( [maximum depth reached] ) ) ) ) $c = array( 'id' => '003879', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'Abdulrahman S. Attiyat and Gary D. Christian', 'title' => 'Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', 'journal' => 'Anal. Chim. Acta', 'journal_id' => '0584', 'fadid' => 'ANCA1979V0106P00225', 'year' => '1979', 'volume' => '106', 'issue' => '2', 'startpage' => '225', 'endpage' => '231', 'type' => 'Journal Article', 'analytes' => ';1103;2414;', 'matrices' => '', 'techniques' => ';0013;0046;0072;', 'keywords' => ';0147;', 'abstract' => 'Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorase-catalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 x 10^-5 - 6 x 10^-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '6', 'urlcheck' => '2014-10-11 15:40:48', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1016/S0003-2670(01)85005-4', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', Anal. Chim. Acta, 1979 106(2) 225-231', 'firstchar' => 'B', 'twochars' => 'Bi', 'CitationsTechnique' => array( 'id' => '000561', 'citation_id' => '003879', 'technique_id' => '0072' ), 'Analyte' => array( (int) 0 => array( 'id' => '01103', 'name' => 'Glycerol', 'iupac_name' => 'propane-1,2,3-triol', 'casrn' => '56-81-5', 'synonyms' => '1,2,3-propanetriol; 1,2,3-Trihydroxypropane; D-glycerol; glycyl alcohol; Glycerin; Glycerin mist; glyceritol; Glycerol; L-glycerol; Polyhydric alcohols; Propanetriol; trihydroxypropane', 'total' => '41', 'inchi' => 'InChI=1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2', 'inchikey' => 'PEDCQBHIVMGVHV-UHFFFAOYSA-N', 'formula' => 'C3H8O3', 'oxstate' => '', 'url' => '', 'charge' => '0', 'class1' => 'Organic compound', 'class2' => 'NA', 'class3' => 'NA', 'class4' => 'Molecule', 'class5' => '', 'isgroup' => '', 'checked' => 'yes', 'citation_count' => '0', 'updated' => '2015-10-23 09:59:11', 'first' => 'G', 'nametotal' => 'Glycerol**41', 'AnalytesCitation' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '02414', 'name' => 'Triglycerides', 'iupac_name' => '', 'casrn' => '', 'synonyms' => '', 'total' => '12', 'inchi' => 'InChI=1S/C6H8O6/c7-3-10-1-6(12-5-9)2-11-4-8/h3-6H,1-2H2', 'inchikey' => '', 'formula' => '', 'oxstate' => null, 'url' => '', 'charge' => null, 'class1' => '', 'class2' => '', 'class3' => '', 'class4' => '', 'class5' => '', 'isgroup' => 'no', 'checked' => 'no', 'citation_count' => '0', 'updated' => '2015-12-11 09:53:17', 'first' => 'T', 'nametotal' => 'Triglycerides**12', 'AnalytesCitation' => array( [maximum depth reached] ) ) ), 'Matrix' => array(), 'Keyword' => array( (int) 0 => array( 'id' => '0147', 'type' => 'Reagent', 'keyword' => 'Enzyme', 'newKeyword' => '', 'synonyms' => '', 'fao' => '', 'total' => '276', 'first' => 'E', 'keytotal' => 'Enzyme**276', 'CitationsKeyword' => array( [maximum depth reached] ) ) ) ) $i = (int) 0 $path = '' $a = '' $url = 'http://dx.doi.org/10.1016/S0003-2670(01)85005-4' $aus = 'Abdulrahman S. Attiyat and Gary D. Christian'include - APP/View/Elements/citation.ctp, line 40 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Techniques/view.ctp, line 52 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 968 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 109
Abstract:
Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorase-catalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 x 10^-5 - 6 x 10^-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique.
Glycerol
Triglycerides
Enzyme
"Continuous-flow Determination Of Blood Alcohol Using Biamperometric Monitoring Of Enzymatic Reaction"
Anal. Lett.
1987 Volume 20, Issue 7 Pages 1099-1113
Notice (8): Undefined variable: uid [APP/View/Elements/citation.ctp, line 40]Attiyat, A.S.;Christian, G.D.Code Context?><?phpif($uid!='') {$viewFile = '/home/stuchalk/public_html/fad/app/View/Elements/citation.ctp' $dataForView = array( 'data' => array( 'Technique' => array( 'id' => '0072', 'label' => 'Electrode', 'level1' => 'Electrode', 'level2' => 'carbon', 'level3' => 'open tubular', 'level4' => '', 'level5' => '', 'synonyms' => '', 'champ' => '', 'total' => '2', 'updated' => '0000-00-00 00:00:00', 'name' => 'Electrode, carbon, open tubular', 'nametotal' => 'Electrode, carbon, open tubular**2', 'first' => 'E' ), 'Citation' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'c' => array( 'id' => '009580', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'NA', 'title' => 'Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', 'journal' => 'Anal. Lett.', 'journal_id' => '0820', 'fadid' => 'ANLE1987V0020P01099', 'year' => '1987', 'volume' => '20', 'issue' => '7', 'startpage' => '1099', 'endpage' => '1113', 'type' => 'Journal Article', 'analytes' => ';0931;', 'matrices' => ';0131;', 'techniques' => ';0013;0072;', 'keywords' => ';0258;', 'abstract' => 'A three-channel flow system was used, the first channel pumping NAD+ and dihydrolipoamide dehydrogenase(I), the second alcohol dehydrogenase(II) and K3Fe(CN)6 and the third diluted blood samples, all at the same flow rate. The three channels merged at a single point, thence passing through a flow cell containing two open-tubular carbon electrodes, with a potential difference of 200 mV. In the presence of ethanol and II, NAD+ was reduced to NADH; this was oxidized back to NAD+ by Fe(CN)63- in the presence of I. The Fe(CN)64- formed was detected at the electrodes. Blood samples were initially diluted 100-fold with 0.1 M Tris buffer (pH 8.5). The detection limit was 0.15 µg mL-1 of ethanol in blood and coefficient of variation were 4%. On eleven samples, correlation with a GC method was good (r = 0.98).', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '2007-03-29 17:18:00', 'hits' => '3', 'urlcheck' => '2014-10-12 00:06:26', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1080/00032718708064594', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', Anal. Lett., 1987 20(7) 1099-1113', 'firstchar' => 'C', 'twochars' => 'Co', 'CitationsTechnique' => array( 'id' => '007949', 'citation_id' => '009580', 'technique_id' => '0072' ), 'Analyte' => array( (int) 0 => array( [maximum depth reached] ) ), 'Matrix' => array( (int) 0 => array( [maximum depth reached] ) ), 'Keyword' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'i' => (int) 1 ) $data = array( 'Technique' => array( 'id' => '0072', 'label' => 'Electrode', 'level1' => 'Electrode', 'level2' => 'carbon', 'level3' => 'open tubular', 'level4' => '', 'level5' => '', 'synonyms' => '', 'champ' => '', 'total' => '2', 'updated' => '0000-00-00 00:00:00', 'name' => 'Electrode, carbon, open tubular', 'nametotal' => 'Electrode, carbon, open tubular**2', 'first' => 'E' ), 'Citation' => array( (int) 0 => array( 'id' => '003879', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'Abdulrahman S. Attiyat and Gary D. Christian', 'title' => 'Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', 'journal' => 'Anal. Chim. Acta', 'journal_id' => '0584', 'fadid' => 'ANCA1979V0106P00225', 'year' => '1979', 'volume' => '106', 'issue' => '2', 'startpage' => '225', 'endpage' => '231', 'type' => 'Journal Article', 'analytes' => ';1103;2414;', 'matrices' => '', 'techniques' => ';0013;0046;0072;', 'keywords' => ';0147;', 'abstract' => 'Biamperometric measurements, with open tubular carbon electrodes in flowing streams, are used to monitor the concentration of hexacyanoferrate(II) produced by the diaphorase-catalyzed reaction between NADH and hexacyanoferrate(III). NADH produced by the glycerol dehydrogenase-catalyzed reaction between glycerol and NAD can be measured in this manner to determine glycerol concentrations; linear calibration plots are obtained in the range 5 x 10^-5 - 6 x 10^-1 M glycerol. Triglycerides are hydrolyzed by lipase and the glycerol liberated is measured similarly. Glycerol and triglycerides are determined in serum control samples by the standard addition technique.', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '0000-00-00 00:00:00', 'hits' => '6', 'urlcheck' => '2014-10-11 15:40:48', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1016/S0003-2670(01)85005-4', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Biamperometric determination of glycerol and triglycerides with open tubular carbon electrodes in flow streams', Anal. Chim. Acta, 1979 106(2) 225-231', 'firstchar' => 'B', 'twochars' => 'Bi', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array([maximum depth reached]), 'Keyword' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '009580', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'NA', 'title' => 'Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', 'journal' => 'Anal. Lett.', 'journal_id' => '0820', 'fadid' => 'ANLE1987V0020P01099', 'year' => '1987', 'volume' => '20', 'issue' => '7', 'startpage' => '1099', 'endpage' => '1113', 'type' => 'Journal Article', 'analytes' => ';0931;', 'matrices' => ';0131;', 'techniques' => ';0013;0072;', 'keywords' => ';0258;', 'abstract' => 'A three-channel flow system was used, the first channel pumping NAD+ and dihydrolipoamide dehydrogenase(I), the second alcohol dehydrogenase(II) and K3Fe(CN)6 and the third diluted blood samples, all at the same flow rate. The three channels merged at a single point, thence passing through a flow cell containing two open-tubular carbon electrodes, with a potential difference of 200 mV. In the presence of ethanol and II, NAD+ was reduced to NADH; this was oxidized back to NAD+ by Fe(CN)63- in the presence of I. The Fe(CN)64- formed was detected at the electrodes. Blood samples were initially diluted 100-fold with 0.1 M Tris buffer (pH 8.5). The detection limit was 0.15 µg mL-1 of ethanol in blood and coefficient of variation were 4%. On eleven samples, correlation with a GC method was good (r = 0.98).', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '2007-03-29 17:18:00', 'hits' => '3', 'urlcheck' => '2014-10-12 00:06:26', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1080/00032718708064594', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', Anal. Lett., 1987 20(7) 1099-1113', 'firstchar' => 'C', 'twochars' => 'Co', 'CitationsTechnique' => array( [maximum depth reached] ), 'Analyte' => array( [maximum depth reached] ), 'Matrix' => array( [maximum depth reached] ), 'Keyword' => array( [maximum depth reached] ) ) ) ) $c = array( 'id' => '009580', 'authors' => 'Attiyat, A.S.;Christian, G.D.', 'authorsweb' => 'NA', 'title' => 'Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', 'journal' => 'Anal. Lett.', 'journal_id' => '0820', 'fadid' => 'ANLE1987V0020P01099', 'year' => '1987', 'volume' => '20', 'issue' => '7', 'startpage' => '1099', 'endpage' => '1113', 'type' => 'Journal Article', 'analytes' => ';0931;', 'matrices' => ';0131;', 'techniques' => ';0013;0072;', 'keywords' => ';0258;', 'abstract' => 'A three-channel flow system was used, the first channel pumping NAD+ and dihydrolipoamide dehydrogenase(I), the second alcohol dehydrogenase(II) and K3Fe(CN)6 and the third diluted blood samples, all at the same flow rate. The three channels merged at a single point, thence passing through a flow cell containing two open-tubular carbon electrodes, with a potential difference of 200 mV. In the presence of ethanol and II, NAD+ was reduced to NADH; this was oxidized back to NAD+ by Fe(CN)63- in the presence of I. The Fe(CN)64- formed was detected at the electrodes. Blood samples were initially diluted 100-fold with 0.1 M Tris buffer (pH 8.5). The detection limit was 0.15 µg mL-1 of ethanol in blood and coefficient of variation were 4%. On eleven samples, correlation with a GC method was good (r = 0.98).', 'language' => 'English', 'updated' => '2020-12-28 11:25:15', 'sjccheck' => 'Yes', 'sjccheckdate' => '2007-03-29 17:18:00', 'hits' => '3', 'urlcheck' => '2014-10-12 00:06:26', 'urlcheckcode' => 'HTTP/1.1 302 Found', 'pauthor_id' => '00090', 'pauthor' => '!Christian, G.D.', 'address' => 'pau', 'email' => 'pau', 'notes' => null, 'url' => '10.1080/00032718708064594', 'urltype' => 'doi', 'gotpdf' => 'yes', 'partial' => 'no', 'notanalyte' => '', 'citation' => ''Continuous-flow determination of blood alcohol using biamperometric monitoring of enzymatic reaction', Anal. Lett., 1987 20(7) 1099-1113', 'firstchar' => 'C', 'twochars' => 'Co', 'CitationsTechnique' => array( 'id' => '007949', 'citation_id' => '009580', 'technique_id' => '0072' ), 'Analyte' => array( (int) 0 => array( 'id' => '00931', 'name' => 'Ethanol', 'iupac_name' => 'ethanol', 'casrn' => '64-17-5', 'synonyms' => 'alcohol; alcohol dehydrated; algrain; Anhydrol; cologne spirit; cologne spirits (alcohol); Denatured alcohol; Ethyl alcohol; Ethanol; ethanol 200 proof; Ethanol absolute; ethyl hydrate; ethyl hydroxide; fermentation alcohol; grain alcohol; jaysol; jaysol s; methylcarbinol; molasses alcohol; potato alcohol; sd alcohol 23-hydrogen; spirit; spirits of wine; Synasol; tecsol', 'total' => '115', 'inchi' => 'InChI=1S/C2H6O/c1-2-3/h3H,2H2,1H3', 'inchikey' => 'LFQSCWFLJHTTHZ-UHFFFAOYSA-N', 'formula' => 'C2H6O', 'oxstate' => 'Zero', 'url' => '', 'charge' => '0', 'class1' => 'Organic compound', 'class2' => 'NA', 'class3' => 'NA', 'class4' => 'Molecule', 'class5' => '', 'isgroup' => '', 'checked' => 'yes', 'citation_count' => '0', 'updated' => '2015-10-23 09:57:39', 'first' => 'E', 'nametotal' => 'Ethanol**115', 'AnalytesCitation' => array( [maximum depth reached] ) ) ), 'Matrix' => array( (int) 0 => array( 'id' => '0131', 'label' => 'Whole ', 'level1' => 'Biological fluid', 'level2' => 'blood', 'level3' => 'whole', 'level4' => '', 'level5' => '', 'synonyms' => '', 'total' => '41', 'url' => '', 'updated' => '2015-12-08 19:41:10', 'name' => 'Biological fluid, blood, whole', 'nametotal' => 'Biological fluid, blood, whole**41', 'first' => 'B', 'CitationsMatrix' => array( [maximum depth reached] ) ) ), 'Keyword' => array( (int) 0 => array( 'id' => '0258', 'type' => 'Chemometrics', 'keyword' => 'Method comparison', 'newKeyword' => '', 'synonyms' => '', 'fao' => '', 'total' => '1232', 'first' => 'M', 'keytotal' => 'Method comparison**1232', 'CitationsKeyword' => array( [maximum depth reached] ) ) ) ) $i = (int) 1 $path = '' $a = '' $url = 'http://dx.doi.org/10.1080/00032718708064594' $aus = 'Attiyat, A.S.;Christian, G.D.'include - APP/View/Elements/citation.ctp, line 40 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Techniques/view.ctp, line 52 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 968 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 109
Abstract:
A three-channel flow system was used, the first channel pumping NAD+ and dihydrolipoamide dehydrogenase(I), the second alcohol dehydrogenase(II) and K3Fe(CN)6 and the third diluted blood samples, all at the same flow rate. The three channels merged at a single point, thence passing through a flow cell containing two open-tubular carbon electrodes, with a potential difference of 200 mV. In the presence of ethanol and II, NAD+ was reduced to NADH; this was oxidized back to NAD+ by Fe(CN)63- in the presence of I. The Fe(CN)64- formed was detected at the electrodes. Blood samples were initially diluted 100-fold with 0.1 M Tris buffer (pH 8.5). The detection limit was 0.15 µg mL-1 of ethanol in blood and coefficient of variation were 4%. On eleven samples, correlation with a GC method was good (r = 0.98).
Ethanol
Whole
Method comparison