Contact Info
Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf
Electrode
Classification:
Electrode
-> antigen modified
Citations 1
"Electrochemiluminescence Of Luminol For 2,4-D Optical Immunosensing In A Flow Injection Analysis System"
Sens. Actuat. B
1998 Volume 51, Issue 1-3 Pages 100-106
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Marquette and Lo&iuml;c J. Blum*'include - APP/View/Elements/citation.ctp, line 40 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::_renderElement() - CORE/Cake/View/View.php, line 1224 View::element() - CORE/Cake/View/View.php, line 418 include - APP/View/Techniques/view.ctp, line 52 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 968 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 200 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 109
Abstract:
Luminol-labeled antibodies have been prepared using glutaraldehyde as a crosslinking agent and used in a 2,4-dichlorophenoxyacetic acid (2,4-D) competitive electrochemiluminescent immunosensor. 2,4-D was covalently immobilized at a glassy C electrode surface, via a C6 spacer arm, by a novel procedure giving stable immobilized antigens that could be then stored dry, used, and regenerated 50 times without loss of binding capacity. The luminol electrochemiluminescence detection was performed in a flow injection analysis system. The optimum conditions were an oxidation potential of +500 mV vs. a Pt pseudo-ref. electrode, in the presence of 600 µM H2O2. In these conditions, luminol could be detected in the range 5.5 fmol - 55 nmol. Luminol-labeled anti-2,4-D antibodies or peroxidase-labeled secondary antibodies were tested for the 2,4-D immunodetection. With both the corresponding electrochemiluminescent and chemiluminescent immunoassays it was possible to detect 0.2 µg free 2,4-D/L. The overall time of experiment was 50 min and a linear range of 0.2 µg/L - 200 mg/L was obtained with the peroxidase format, whereas the range was 0.2-200 µg/L with the luminol format.
2,4-dichlorophenoxyacetic acid
Luminol
Apparatus
Detector
Optimization