University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Electrode

Classification: Electrode -> antigen modified

Citations 1

"Electrochemiluminescence Of Luminol For 2,4-D Optical Immunosensing In A Flow Injection Analysis System"
Sens. Actuat. B 1998 Volume 51, Issue 1-3 Pages 100-106

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Christophe A. Marquette and Loïc J. Blum*

Abstract: Luminol-labeled antibodies have been prepared using glutaraldehyde as a crosslinking agent and used in a 2,4-dichlorophenoxyacetic acid (2,4-D) competitive electrochemiluminescent immunosensor. 2,4-D was covalently immobilized at a glassy C electrode surface, via a C6 spacer arm, by a novel procedure giving stable immobilized antigens that could be then stored dry, used, and regenerated 50 times without loss of binding capacity. The luminol electrochemiluminescence detection was performed in a flow injection analysis system. The optimum conditions were an oxidation potential of +500 mV vs. a Pt pseudo-ref. electrode, in the presence of 600 µM H2O2. In these conditions, luminol could be detected in the range 5.5 fmol - 55 nmol. Luminol-labeled anti-2,4-D antibodies or peroxidase-labeled secondary antibodies were tested for the 2,4-D immunodetection. With both the corresponding electrochemiluminescent and chemiluminescent immunoassays it was possible to detect 0.2 µg free 2,4-D/L. The overall time of experiment was 50 min and a linear range of 0.2 µg/L - 200 mg/L was obtained with the peroxidase format, whereas the range was 0.2-200 µg/L with the luminol format.
2,4-dichlorophenoxyacetic acid Luminol Apparatus Detector Optimization