University of North Florida
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Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Xian-En Zhang

Abbrev:
Zhang, X.E.
Other Names:
Address:
Wuhan Institute of Virology, Chinese Academy of Sciences, PR China.
Phone:
0086 27 7641492
Fax:
0086 27 7885422

Citations 3

"Simultaneous Determination Of Maltose And Glucose Using A Dual-electrode Flow Injection System"
Food Chem. 1995 Volume 52, Issue 2 Pages 187-192
Hong-Bo Qu, Xian-En Zhang* and Shu-Zheng Zhang

Abstract: Glucose oxidase (GOD) membranes were prepared by dropping 3 µL of 20% BSA and 2 µL of 2.5% glutaraldehyde solution on to a porous Teflon membrane where they were mixed, spread and left to stand for 30 min at 30°C. The amyloglucosidase/glucose oxidase (A/G) membrane was prepared in the same way but with 5 µL of amyloglucosidase mixed with 6 µL glutaraldehyde solution. The membranes were covered with a dialysis membrane and fixed over an O2 electrode tip to form part of a FIA system (diagram given); 25 µL of the sample was injected into the water flow stream (2.86 mL/min) at 37±0.05°C and the electrode output was recorded. Calibration graphs were linear from 0.3-30 mM/l of sugar with RSD of 3.2% and 1.6% for the GOD and A/G electrode, respectively. Highly active enzyme membranes were made which showed no decay in 20 days. The method was comparable with the Fehling titration method and could be used for online monitoring and control in industrial processes.
Glucose Maltose Food Electrode Electrode Process control Dialysis

"Disposable Screen-printed Enzyme Sensor For Simultaneous Determination Of Starch And Glucose"
Biotechnol. Techniq. 1999 Volume 13, Issue 6 Pages 359-362
Tao Hu, Xian-En Zhang, Zhi-Ping Zhang

Abstract: A disposable screen-printed biosensor with dual working electrodes was first established for simultaneous determination of starch and glucose. The electrochemical behavior of the sensor was assessed using cyclic voltammetry and chronoamperometry. The linear response ranges of the sensor were up to 0.4% (w/v) starch and 20 mmol glucose L-1. Real samples were analyzed using the proposed method and the reference method and the correlation coefficient was 0.997.

"DNA Optical Sensor: A Rapid Method For The Detection Of DNA Hybridization"
Biosens. Bioelectron. 1998 Volume 13, Issue 3-4 Pages 451-458
Xiao Chen, Xian-En Zhang*, Yi-Quan Chai, Wei-Ping Hu, Zhi-Phing Zhang, Xiao-Mei Zhang and Anthony E. G. Cass

Abstract: A DNA optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of DNA hybridization. Bacterial alkaline phosphatase (phoA) gene and Hepatitis B virus (HBV) DNA were used as target DNA. A biotinylated DNA probe was used to capture the target gene onto the streptavidin-coated magnetic beads and a calf intestine alkaline phosphatase (CAP)-labelled DNA probe was used for subsequent enzymatic chemiluminescence detection. The detection cycle was less than 30 min, excluding the DNA hybridization time, which was about 100 min. Both the phoA gene and HBV DNA could be detected at picogramme or femtomole level. No response signal was obtained when target DNA did not exist in the sample. Successive sample detection could be made by removing the magnetic field and a washing step. (27 References)
DNA DNA, hepatitis B virus Chemiluminescence Magnetic particles