University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Brian W. Renoe

Abbrev:
Renoe, B.W.
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Departments of Pathology and Chemistry, University of Virginia Medical Center, Charlottesville, VA 22908 USA
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Citations 3

"Use Of A Flow Injection Sample Manipulator As An Interface Between A High-Performance Liquid Chromatograph And An Atomic Absorption Spectrophotometer"
Clin. Chem. 1981 Volume 27, Issue 9 Pages 1546-1550
BW Renoe, CE Shideler and J Savory

Abstract: We describe an integrated, molecular-absorbance, atomic absorption instrument for studying metal/ligand binding in clinical samples. For an interface between the 'high performance' liquid chromatograph and the atomic absorption instrument we used a flow injection sample manipulator, thus allowing both the chromatograph and the atomic absorption detector to operate at their separate optimum conditions. After specimen separation with a gel permeation column, we measured the molecular components of the column eluate by molecular absorbance spectrometry and the atomic components (calcium and magnesium) by flame atomic absorption spectrophotometry. This instrument system is capable of separating and analyzing multiple components within 20 min of injection of the sample on the column. The chromatograms presented demonstrate the utility of the system for investigating metal binding to a variety of ligands in clinical samples.
Calcium Magnesium Biological material Clinical analysis Spectrophotometry HPLC Complexation Injector Interface

"Automated Multiple Flow Injection Analysis In Clinical Chemistry. Determination Of Total Protein With Biuret Reagent"
Clin. Chem. 1980 Volume 26, Issue 10 Pages 1454-1458
CE Shideler, KK Stewart, J Crump, MR Wills, J Savory and BW Renoe

Abstract: We have examined the feasibility of the automated multiple flow injection technique for application to clinical chemistry by adapting to this system the biuret method for the determination of total protein. Samples were discretely and rapidly introduced into a continuously flowing, nonsegmented reagent stream by means of an automatic sampler and high-pressure injection valve. Pumps operating at 1380-2070 kPa (200-300 psi) were utilized to introduce the biuret reagent and saline diluent into the system separately at flow rates of 72 and 47 µL/s, respectively. Use of 20 µL sample and a 3.0 s reaction-delay coil was adequately sensitive for analysis for total protein by this method. Samples were analyzed at a rate of 150/h with no detectable between-sample carryover. Within-run precision studies yielded relative standard deviations of 2.5% and less. Total protein values obtained by this method correlated well with those obtained by centrifugal analyzer and bubble-segmented continuous-flow biuret methods.
Albumins Proteins Blood Serum Clinical analysis Spectrophotometry Standard method Method comparison

"Automated Multiple Flow Injection Analysis In Clinical Chemistry. Determination Of Albumin With Bromocresol Green"
Clin. Chem. 1980 Volume 26, Issue 2 Pages 331-334
BW Renoe, KK Stewart, GR Beecher, MR Wills and J Savory

Abstract: We describe an adaptation of automated multiple flow injection analysis instrumentation to an analysis for albumin in serum. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative standard deviation of less than 1%, good correlations with published procedures, and no measurable interferences. The simplicity and flexibility of the instrumentation and its performance integrity, as indicated by the analytical results, make this a viable clinical chemical tool. An adaptation of automated multiple flow injection analysis instrumentation to an analysis for albumin in (human) serum is described. The bromcresol green reaction was used to test the utility of the system. The approach yielded albumin results with excellent sensitivity, no measurable carryover, a relative SD of less than 1%, good correlations with published procedures and no measurable interferences. The simplicity and flexibility of the instrumentation and its performance integrity, as indicated by the analytical results, make this a viable clinical chemical tool.
Albumin Serum Human Clinical analysis Spectrophotometry Interferences Method comparison