University of North Florida
Browse the Citations
-OR-

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

View Stuart Chalk's profile on LinkedIn

Cees Gooijer

Abbrev:
Gooijer, C.
Other Names:
Address:
Laser Center, Department of Analytical Chemistry and Applied Spectroscopy, Vrije Universiteit Amsterdam, de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
Phone:
+31 20 4447540
Fax:
+31 20 4447543

Citations 7

"On The Mechanism Of Peroxyoxalate Chemiluminescence. Quenched Chemiluminescence As A Detection Method In HPLC"
J. Biolumin. Chemilumin. 1989 Volume 4, Issue 1 Pages 479-483
C. Gooijer*, P. van Zoonen, N. H. Velthorst, R. W. Frei

Abstract: Several analytes such as the inorganic anions bromide, iodide, sulphite and nitrite and organic compounds as substituted anilines and sulphur compounds cause quenching of peroxyoxalate chemiluminescence. A detection method for liquid chromatography based on the quenching phenomenon has been developed. It makes use of an immobilized luminophore, i.e. 3-aminofluoranthene covalently bound via an alkyl-spacer on controlled pore glass, packed in the detector cell.The mechanism behind the quenching has been elucidated by investigating the roles of luminophores (both in the liquid and in solid state) and oxalates in peroxylate CL with respect to quenchers. Most probably the quencher destroys the radical ion pair produced after electron transfer in the last stage of the CIEEL reaction scheme, thus preventing the formation of electronically excited luminophore.

"A Flow Injection Kinase Assay System Based On Time-Resolved Fluorescence Resonance Energy-Transfer Detection In The Millisecond Range"
Anal. Chem. 2004 Volume 76, Issue 15 Pages 4292-4298
Junko Hirata, Camiel F. de Jong, Maarten M. van Dongen, Joost Buijs, Freek Ariese, Hubertus Irth and Cees Gooijer

Abstract: A flow injection analysis (FIA) system for biochemical assays using time-resolved fluorescence resonance energy transfer (TR-FRET) in the millisecond time scale was developed. As a model system, we studied a kinase assay, measuring the phosphorylation of poly(GT)-biotin (substrate) by a receptor tyrosine kinase (epidermal growth factor receptor). A streptavidin labeled with XL665 (SA-XL665)-the acceptor-was coupled to the biotin moiety, and an antiphosphotyrosine antibody labeled with europium cryptate (Ab-EuK)-the donor-was coupled to the phosphorylated tyrosine group(s). Long-lived FRET can only occur if the substrate is successfully phosphorylated. For the time-resolved detection of such long-lived luminescence phenomena in a flow system, the repetition rate of the excitation source plays a crucial role. Good results were obtained for a small-sized commercially available quadrupled Nd:YAG laser emitting at 266 nm with a repetition rate of 7.8 kHz and a pulse width of 0.3 ns. The long-lived emissions of the donor at 625 nm and that of the acceptor at 665 nm were monitored simultaneously with two photomultipliers, using a delay time of 50 µs and a gate time of 75 µs to exclude background fluorescence interferences. In the FIA experiments, the Ab-EuK concentration was 6 nM and the substrate concentration and SA-XL665 concentrations were 7 nM. By monitoring the intensity changes at 625 and 665 nm, the inhibition of tyrosine kinase by tyrphostin AG1478 was studied and an IC50 value of 5.1 ± 0.4 nM obtained.

"Capillary Electrophoresis Coupled On-Line With Ultraviolet Resonance Raman Spectroscopy"
Anal. Chem. 2003 Volume 75, Issue 21 Pages 5697-5702
Reyer J. Dijkstra, Evtim V. Efremov, Freek Ariese, Udo A. Th. Brinkman and Cees Gooijer

Abstract: Capillary electrophoresis (CE) and resonance Raman spectroscopy (RRS) with excitation in the deep ultraviolet (UV) region (λex: 244 or 257 nm) were coupled on-line. The potential of this hyphenated technique, denoted as CE-UV-RRS, for analyte confirmation/identification purposes was explored with aromatic sulfonic acids and nucleotides as test compounds. Good-quality UV-RRS spectra could be recorded on-the-fly. Identification limits for the nucleotides were in the 10^-125 µg/mL range. The RRS spectra showed sufficient characteristic features to enable analyte confirmation. In addition, the identification power of UV-RRS was studied with substituted pyrenes as model compounds. The compounds were distinguishable on the basis of their RRS spectra at 244 nm.

"Quenched Peroxyoxalate Chemiluminescence As A New Detection Principle In Flow Injection Analysis And Liquid Chromatography"
Anal. Chem. 1986 Volume 58, Issue 6 Pages 1245-1248
Piet Van Zoonen, Dik A. Kamminga, Cees Gooijer, Nel H. Velthorst, and Roland W. Frei

Abstract: The chemiluminescence method for H2O2, which involves solid bis-(2,4,6-trichlorophenyl) oxalate(I) packed into a cell together with 3-aminofluoranthene immobilized on porous glass beads (CPG-10), is studied. The H2O2 monitor system is as previously described (Anal. Abstr., 1986, 48, 6H41) and the block diagram of the detector is shown. The mobile phase for both flow injection analysis and HPLC is acetonitrile - 0.05 M Tris buffer, pH 8 (4:1) 1 mM in H2O2. Oxidizable compounds, e.g., anilines, NO2- and SO32-, influence the sensitivity of the I chemiluminescence method for H2O2 but do not affect the rectilinear relationship. The quenched peroxyoxalate chemiluminescence method has potential as a detection method for these compounds.
Hydrogen peroxide Chemiluminescence HPLC Porous glass beads Immobilized enzyme Quenching

"Quenched Room Temperature Phosphorescence Detection For Flow Injection And Liquid Chromatography"
Anal. Chem. 1983 Volume 55, Issue 12 Pages 1886-1893
J. J. Donkerbroek, A. C. Veltkamp, C. Gooijer, N. H. Velthorst, and R. W. Frei

Abstract: The cited detection technique is based on quenching of the phosphorescence of biacetyl by suitable analytes. The sensitivity of such quenching is dependent on the bimolecular rate constant of the quenching reaction. Values for this constant and for the estimated limit of detection in aqueous 83.7% acetonitrile, water or hexane are listed for various chloroanilines, amines, aromatic N compounds, S-containing compounds, chlorophenols, aromatic and aliphatic hydroxy-compounds, and inorganic ions. The detection limits are often very low, e.g., 1 to 10 nM. The rectilinearity of the relationship between signal and concentration. can be extended by use of electronic signal inversion techniques. Application of this detection method to flow injection analysis and HPLC is demonstrated.
Anilines, chloro Amines Phenols, chloro Anions, inorganic Clinical analysis HPLC Phosphorescence Quenching Review Theory

"Column Liquid Chromatography With Diode Laser-induced Fluorescence Detection Of Carboxylic Acids After Pre-column Derivatisation"
Anal. Chim. Acta 1995 Volume 315, Issue 1-2 Pages 209-216
Arjan J. G. Mank, Marco C. Beekman, Nel H. Velthorst, Udo A. Th. Brinkman, Henk Lingeman and Cees Gooijer*

Abstract: Several pre-column approaches to fluorescence derivatization of carboxylic acids, compatible with diode laser-induced fluorescence (DIO-LIF) detection, were compared. For this purpose red-absorbing labels containing hydrazide, bromoacetamide and iodoacetamide functionalities were studied. The sensitivity of DIO-LIF detection is illustrated by the (3-10) x 10^-12 M (75-250 amol) detection limit for various derivatised carboxylic acids. The real-life detection limit for the carboxylic acids is 3 x 10 -8 M, because derivatization is not quantitative at lower concentrations. Derivatised n-alkylcarboxylic acids and non-steroidal anti-inflammatory drugs are easily separated by column liquid chromatography, despite the attachment of the bulky red-absorbing labels. The determination of the anti-inflammatory drug naproxen in saliva is shown as an example.
Carboxylic acids HPLC Fluorescence Pre-column derivatization Laser diode

"Flow Injection System For Determination Of Singlet Oxygen Quenching Efficiencies Utilizing Online Dioxetane Chemiluminescence Detection"
Anal. Chim. Acta 1994 Volume 290, Issue 1 Pages 201-214
H. A. G. Niederländer, M. M. de Jong, C. Gooijer* and N. H. Velthorst

Abstract: The flow injection method was based on the photochemical generation of singlet oxygen which reacted online with 1,2-diethoxyethene to form 3,4-diethoxy-1,2-dioxetane (I). I was detected by chemiluminescence (CL) following reaction with 9,10-dibromoanthracene-2-sulfonate in the detector cell at 70°C. The injection of a quencher resulted in a decrease in CL intensity. The method was used to determine the singlet oxygen quenching rate constants for a variety of quenchers including sulfur compounds, amines and phenols. The values obtained were in good agreement with literature values. The method was fast (up to 15 samples/h) and a large variety of solvents could be used.
Oxygen Chemiluminescence Quenching