Abstract: The behavior of a complex formed between Cu and glycine was studied on Polysphere RP C18, HEMA RP C18 and Superpac-Spherisorb ODS-2 Dual Bond stationary phases. The best results were obtained using Superpac Dual Bond, as the Cu-glycine complex did not decompose as it did with the other stationary phases tested. The Cu-glycine complex was determined by a post-column derivatization procedure using o-phthalaldehyde at 50°C with eluent flow rate of 1-1.5 ml/min, derivatization reagent flow rate of 0.5 ml/min and fluorimetric detection at 530 nm (excitation 360 nm). The detection limit was 0.1 fmol. The method was applied to the determination of amino-acids.