University of North Florida
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Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Tomasz Kleszczewski

Abbrev:
Kleszczewski, T.
Other Names:
Address:
Institute of Biophysics, Medical Academy, Mickiewicza 2A, 15-230 Bialystok, Poland
Phone:
+48-85-745-7580
Fax:
+48-85-745-7581

Citations 2

"Flow Injection Spectrophotometric Determination Of L-ascorbic Acid In Biological Matters"
J. Pharm. Biomed. Anal. 2002 Volume 29, Issue 4 Pages 755-759
Tomasz Kleszczewski and Ewa Kleszczewska

Abstract: A method for spectrophotometric determination Of L-ascorbic acid (vitamin Q in tissues by flow injection analysis is reported. The procedure is based on the oxidation of analyze with iron(III) and 2,2-dipyridyl. Concentrations of vitamin C in the range of 0.5-20 ppm have been determined with a relative standard deviation 1.2% (n = 15). The injection rate was 40 samples per h. The method was applied to the determination Of L-ascorbic acid in rats tissues (blood serum, brain, and liver) and compared favourably with an independent reference method based on spectrophotometry. (C) 2002 Published by Elsevier Science B.V.
Ascorbic acid

"FIA Of Vitamin C In Blood Serum In Humans At Increasing Ethanol Concentration"
J. Pharm. Biomed. Anal. 2001 Volume 25, Issue 3-4 Pages 477-481
Tomasz Kleszczewski and Ewa Kleszczewska

Abstract: The evaluation of the ethanol influence on the changes in vitamin C levels has been done in humans at increasing ethanol concentration. Flow injection analysis method (FIA) with the spectrophotometric detection was used to determine L-ascorbic acid levels. The procedure is based on the oxidation of the analysis with iron (III) and 2,2 -dipyrydyl. Concentrations of vitamin C in the range of 0.4-1.3 ppm have been determined with a relative standard deviation (RSD) 1.2% (n = 15). The injection rate was 40 samples h-1. The comparison of L-ascorbic acid levels in humans showed a statistical difference at P < 0.05 in the analyzed group. (C) 2001 Elsevier Science B.V. All rights reserved.
Ascorbic acid