Abstract: A review of thermistor-based calorimetric measurement is presented. The principles of thermometric measurements are highlighted in the introduction followed by the instrumentation, materials and methods. Various applications relating to enzyme activity measurements, clinical monitoring, process monitoring, multianalyte determination, hybrid sensing, environmental monitoring, non-aqueous measurements and other miscellaneous applications are described. A brief note on future developments and a detailed reference list is also included. (C) 2001 Elsevier Science B.V. All rights reserved.

Abstract: The stability of horseradish peroxidase (HRP) in aqueous and organic solvents is applied to develop a simple thermometric procedure to detect the binding of retinoic acid-HRP conjugate to retinol binding protein (RBP). Butanone peroxide (BP) in organic phase and hydrogen peroxide in aqueous phase is detected thermometrically on a HRP column, immobilized by cross-linking with glutaraldehyde on controlled pore glass (CPG). Acetone, acetonitrile, methanol, and 2-butanol are used for detection of BP, in the flow injection analysis (FIA) mode. A linear range between 1 and 50 mM BP is obtained in all the organic solvents with a precision of 5-7% (CV%). The magnitude and nature of the thermometric response is significantly different in each organic solvent. The stability of HRP in the organic phase is used to study the stability of a retinoic acid-HRP conjugate bound to immobilized REP. The response of HRP (to 20 mM BP) in the retinoic acid- HRP conjugate is used as an indicator of the stability of the RBP-retinoic acid-HRP complex, after challenges with various organic/aqueous solvents. Both immobilized HRP and REP are stable at least for 6 months. The effect of o-phenylene diamine on the thermometric response of HRP is also investigated. A scheme for the design of a thermometric retinol (vitamin A) biosensor is proposed.