University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Saverio Mannino

Abbrev:
Mannino, S.
Other Names:
Address:
Department of Food Science and Technology, University of Milan, Via Celoria 2, 20133 Milan, Italy
Phone:
NA
Fax:
NA

Citations 8

"Electrochemical Sensor Detecting Free Sulfhydryl Groups: Evaluation Of Milk Heat Treatment"
J. Dairy Sci. 2000 Volume 83, Issue 9 Pages 1933-1938
M. S. Cosio, S. Mannino, and S. Buratti

Abstract: We describe a new and rapid method for the evaluation of reactive sulfhydryl groups in whey proteins obtained after precipitation of casein by acetic acid at pH 4.6. The procedure is based on the use of a wire tungsten electrode operating at -0.2 V versus saturated calomel electrode in flow injection analysis. The method was applied to raw milks and to commercial pasteurized and UHT milks. Results showed that the tungsten electrode constituted a robust amperometric sensor that could be used to differentiate milks that underwent different heat treatments. The decrease of thiol content in the whey proteins from samples was in agreement with the whey protein content found by HPLC. The procedure is suitable for on-line quality control of heat-treated milks.

"Rapid Electrochemical Method For The Evaluation Of The Antioxidant Power Of Some Lipophilic Food Extracts"
J. Agric. Food Chem. 2001 Volume 49, Issue 11 Pages 5136-5141
Susanna Buratti, Nicoletta Pellegrini, Oreste V. Brenna, and Saverio Mannino

Abstract: In this paper, a novel electrochemical method to evaluate the antioxidant power of lipophilic compounds present in vegetables, such as carotenoids, chlorophylls, tocopherols, and capsaicin, is reported. The method is based on a flow injection system with an electrochemical detector equipped with a glassy carbon working electrode operating amperometrically at a potential of + 0.5 V (vs Ag/AgCl). The proposed method is selective for lipophilic compounds having antioxidant power. When applied to pure compounds, the order of antioxidant power resulted as follows: lycopene > β-carotene > zeaxanthin > α-carotene > β-cryptoxanthin > lutein > α-tocopherol > capsaicin > chlorophyll a > chlorophyll b > astaxanthin > canthaxanthin. Results obtained on five vegetable and two fruit extracts were compared to those obtained by the 2,2-azinobis(3-ethylbenz-thiazoline-6-sulfonic) acid (ABTS) radical cation decolorization assay, one of the most used methods to evaluate the total antioxidant capacity of foods. A good correlation between the two methods was found, except for spinach, because of the different antioxidant powers assigned by the two methods to chlorophylls. In conclusion, results suggest that the proposed electrochemical method can be successfully employed for the direct, rapid, and reliable monitoring of the antioxidant power of lipophilic food extracts. [Evaluation Studies, Journal Article; In English; United States]

"Simultaneous Determination Of Glucose And Galactose In Dairy Products By Two Parallel Amperometric Biosensors"
Ital. J. Food Sci. 1999 Volume 11, Issue 1 Pages 57-65
Mannino, S.; Cosio, M. S.; Buratti, S.

Abstract: A sensitive, specific and rapid procedure for the simultaneous determination of glucose and galactose in dairy products based on biosensors is described. The biosensing probes consisted in two electrodes operating in parallel in a Flow Injection Analysis (FIA) system. Both electrodes consisted of a 5% rhodium-on-carbon paste, one equipped with glucose oxidase (GOx) and the other with galactose oxidase (GAOx) and peroxidase (HRP) for the determination of glucose and galactose, respectively. The rhodium-on-carbon paste electrode shows enhanced selectivity (particularly discrimination against ascorbic acid) and electrocatalysis of the reduction at lower potential of hydrogen peroxide produced by the oxidase, The response was linear from 250 to 4,000 mg L-1 with a detection limit of 45 for glucose and 98 mg L-1 for galactose. Compared with classical methods mainly based on HPLC, the proposed procedure features straightforward sample preparation and improved sensitivity and selectivity, All results reported demonstrate that the rhodium-on-carbon paste/ enzyme electrode constitutes a robust amperometric biosensor which can be used for quality control in dairy industries.
Glucose

"Cobalt(II, III)-oxide Chemically Modified Electrode As Amperometric Detector In Flow Injection Systems"
Electroanalysis 1993 Volume 5, Issue 2 Pages 145-148
S. Mannino, M. S. Cosio, S. Ratti

Abstract: A mixture of Co oxides was blended with C paste (graphite powder - oil - oxide; 5:4:1) and packed in the cavity of a flow detector. Constant-potential amperometry in a flow system was used to examine the suitability of the modified electrode for the detection of biologically important substances. The mobile phases were 0.1 M NaOH, 0.1 M phosphate buffer of pH 7 and 0.1 M acetate buffer of pH 5.5 (1.5 mL min-1). The overvoltage for the oxidation of compounds such as glucose and other sugars, H2O2 and cysteine was greatly reduced. The modified electrode was stable, could be easily renewed and gave reproducible results. The responses of 14 compounds at -300 to +600 mV are tabulated. When operated at -300 mV, the electrode could be used for the determination of H2O2 in milk, even in the presence of other naturally reducing substances. Changing the potential to +400 mV allowed the determination of lactose in the same sample.
Cobalt Lactose Glucose Amperometry Electrode

"Computerized Potentiometric Stripping Analysis Using Asyst Software: Application To Lead Determination In Wines And Vinegars"
Electroanalysis 1989 Volume 1, Issue 2 Pages 177-180
Saverio Mannino*, Giuseppe Fregapane, Mariagrazia Bianco

Abstract: A computerized system for potentiometric stripping analysis made of commercially available components with corresponding software is described. Application to the determination of lead in wines and vinegars is also reported.
Lead Wine Food Potentiometric stripping analysis Computer

"Determination Of Ascorbyl 6-palmitate In Food Matrices By Amperometric Flow Injection Analysis"
Analyst 2001 Volume 126, Issue 8 Pages 1466-1468
Susanna Buratti, Maria Stella Cosio, Simona Benedetti and Saverio Mannino

Abstract: In this paper a rapid method based on a FIA (flow injection analysis) system with amperometric detection for the evaluation of ascorbyl 6-palmitate in foods is described. The selectivity of the proposed method is related to the low anodic potential applied to the working glassy carbon electrode (+0.1 V vs. Ag/AgCl) that leaves out interferences from ascorbic acid and phenolic compounds. By flow injection analysis, under optimized conditions. the calibration curve was linear in the range 0-20 mg L-1 and the detection limit was 0.2 mg l-1.

"Evaluation Of The Antioxidant Power Of Olive Oils Based On A FIA System With Amperometric Detection"
Analyst 1999 Volume 124, Issue 7 Pages 1115-1118
Saverio Mannino, Susanna Buratti, Maria Stella Cosio and Nicoletta Pellegrini

Abstract: A new method for the evaluation of the total antioxidant power of olive oils, based on a flow injection analysis system with electrochemical detection, is described. It represents an attractive alternative to the mostly used Rancimat method since it is based on the chemical structure of antioxidants and does not require the manipulation of several parameters, such as temperature and oxygen pressure, to accelerate oil oxidation. The proposed procedure is simple, rapid, allows a throughput of 90 samples h-1 and provides a good precision: an RSD of 3.5% was obtained for caffeic acid at the concentration level of 5 mg L-1 (n = 12). A comparison of the proposed procedure with two other methods (Rancimat method and ABTS(.+) decoloration assay) was performed to investigate the applicability and limitations of the proposed method.

"Potentiometric-stripping Analysis Of Lead And Tin With A Continuous-flow System"
Analyst 1984 Volume 109, Issue 7 Pages 905-907
Saverio Mannino

Abstract: The flow cell is as used by Anderson et al. (cf. Anal. Abstr., 1983, 44, 2J123) and contains mercury-film (on vitreous carbon, prepared in situ) working and counter-electrodes and a silver - AgCl reference electrode. The sample is pumped through the cell at a constant rate and electrolysis is carried out for an appropriate time at -1.2 V. Then de-aerated ammonium citrate buffer solution (pH 4.6) containing 10 ppm of Hg(II) is passed through the cell for 30 s, the flow is stopped and the potentiometric-stripping curve is recorded. Quantitation is by the method of standard additions. The method was applied to eight samples of fruit juices or soft drinks, and results agreed with those by AAS (for Pb) or anodic-stripping voltammetry (for Sn). The proposed method can be used to determine 0.1 ppb of Sn and Pb.
Tin Lead Fruit Beverage Electrode Potentiometric stripping analysis Method comparison Simultaneous analysis Standard additions calibration