University of North Florida
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Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Guenter Gauglitz

Abbrev:
Gauglitz, G.
Other Names:
Address:
Institute of Physical and Theoretical Chemistry, University of TŸbingen, Auf der Morgenstelle 8, 72076 TŸbingen, Germany
Phone:
+49-7071-29-76927
Fax:
+49-7071-29-5490

Citations 6

"Structure And Characterization Of A Micro Flow System For The Study Of Modulated Concentrations"
Trends Anal. Chem. 2001 Volume 20, Issue 4 Pages 186-194
B. Bühler, D. Fröhlich, H. -M. Haake, A. Brecht and G. Gauglitz

Abstract: This article discusses a µflow system that was constructed using the printed circuit board technique, and where fluid flow is by hydrostatic pressure. A new approach in the biosensor field, modulation of analyte concentration, is investigated. The flow and distribution of analyte molecules are examined by observing the dispersion of the analyte molecules under the condition of modulation of their concentration by alternate injection of analyte solution and pure water. A theoretical model for the description of a sequence of dye packages in a µchannel under laminar flow conditions is compared with the experimental data.

"Trends In Biosensors"
GIT Fachz. Lab. 1992 Volume 36, Issue 12 Pages 1253-NA
Gauglitz, G.

Abstract: A summary is presented of developments in biosensors. Topics mentioned briefly include the use of FIA with optical detection; sensors based on enzymatic reactions; optical methods with affinity sensors; miniaturization of pumping systems; fixation and immobilization of biocomponents; the suppression of non-specific reactions; validation of sensors; the use of biomimetric systems; and the use of sensor arrays.
Sensor Review Miniaturization

"Immunoanalytical Techniques For Pesticide Monitoring Based On Fluorescence Detection"
Fresenius J. Anal. Chem. 2000 Volume 366, Issue 6-7 Pages 646-658
U. Schobel, C. Barzen, G. Gauglitz

Abstract: In the field of environmental analysis there is still great potential for development and application of immunoanalytical techniques (IT). Heterogeneous and homogeneous immunoassays (IA), flow injection immunoanalysis (FIIA) and immunosensors (TS) with different detection principles have been developed. In this review we focus on fluorescence methods for pesticide monitoring published since 1992. These techniques offer a high degree of selectivity and, in principle, sensitivity. Restrictions on the limits of detection (LOD) due to background signals are minimized by development of solid-phase separation systems. new fluorescent probes, and new instrumentation.

"Optimized Layer Systems For Immunosensors Based On The RIFS Transducer"
Fresenius J. Anal. Chem. 1994 Volume 349, Issue 5 Pages 360-366
A. Brecht and G. Gauglitz

Abstract: As one approach to direct affinity sensing, a study was made of the use of a transducer, based on thin dielectric films, for reflectometric interference spectroscopy. The transducer system includes a 20-W tungsten white-light source, a sorted bundle of 100 µm optical fibers (17 illuminating, 8 collecting) and a Zeiss-Jena MCS diode-array spectrometer (350-780 nm). For FIA detection film-coated glass substrates are mounted in a 0.3 µL PTFE flow-cell (4.5 x 1.5 mm x 50 µm). Methods to improve the modest detection limits achieved are discussed. Detection of protein coverage down to 10 pg/mm2 was achieved. Applications for direct monitoring of immuno-reactions are being sought.
Protein Biological Spectrophotometry Sensor Interferences

"Low Molecular Weight Analytes In Water By Spectral Interferometry Using A Competitive Immunoassay"
Fresenius J. Anal. Chem. 1994 Volume 348, Issue 8-9 Pages 602-605
G. Lang, A. Brecht and G. Gauglitz

Abstract: Dinitrophenol (I), covalently bound with human serum albumin, was coated onto the surface of a high RI glass fiber and the fiber sensor (10 µL volume) mounted within a flow cell. The fiber surface was additionally blocked to non-specific adsorption using ovalbumin. Solution (100 µg/ml) of monoclonal anti-I in PBS of pH 7.4, were incubated with 5-5000 ng/ml solution of I for 5 min and then pumped through the sensor cell via a flow injection system. The evanescent internal reflectance spectra of I were recorded over 350-780 nm. Calibration graphs of initial response slopes of I with concentration are discussed. A detection limit of 50 ng/ml of I was obtained.
2-Nitrophenol Environmental Immunoassay Electrode Interferences

"Polymer Based RIFS Sensing: An Approach To The Indirect Measurement Of Organic Pollutants In Water"
Fresenius J. Anal. Chem. 1994 Volume 348, Issue 8-9 Pages 598-601
G. Kraus, A. Brecht, V. Vasic and G. Gauglitz

Abstract: Thin polymer films (250 nm-4 µm) of polymethyltrifluoropropylsiloxane (I), polydimethylsiloxane (II) and styrenebutylmethacrylate were fabricated by spin coating high RI glass fibers with 10% solution of the polymers (details given) for use in RIFS (reflectometric interference spectrometry). The sensors (3 m x 25 µL volume) were mounted within a flow cell and water containing toluene (III), 1,2-dichloroethane (IV), trichloroethene (V) and tetrachloroethene (VI) pumped through via a flow injection system at 0.5-0.75 ml/min. The evanescent internal reflectance spectra were recorded over the range 350-780 nm. Calibration graphs of II-coated sensors were linear for 77.2 ng/ml-1.2 mg/ml of IV with a detection limit of 12 ng/ml. The sensitivity of I-coated sensors to III-VI were 14, 3.2, 2.4 and 4 nl/ng, respectively. Sensor response times were typically less than 3 s.
Organic compounds Environmental Spectrophotometry Sensor Interferences Indirect