University of North Florida
Browse the Citations

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

View Stuart Chalk's profile on LinkedIn

Wheat Malt

Classification: Agricultural -> grain -> wheat -> malt

Citations 1

"Flow Injection Spectrophotometric Assay Of α-amylase Activity"
Anal. Chim. Acta 2000 Volume 421, Issue 1 Pages 19-25
J. F. van Staden and L. V. Mulaudzi

Abstract: A spectrophotometric flow injection method for the determination of α-amylase activity is proposed. The method is based on the analysis of the maltose obtained during the hydrolysis of amylose in the presence of α-amylase and relies on the reaction of 3,5 dinitrosalicylic-acid and maltose at boiling temperatures to form a brick red colored complex, monitored spectrophotometrically at 540 nm. The method has proved to be highly sensitive and rapid to allow analysis of 60 samples per hour. The % RSD of this method was found to be <0.5% with the working activity range of 0.005-0.06 FAU. The detection limit was found to be 0.0048 FAU. (FAU: fungal amylase unit; 1 FAU is the amount of the enzyme that hydrolyses 5.26 g of starch per hour. 1 FAU=249.85 SIGMA).
α-Amylase Spectrophotometry Indirect Heated reaction Method comparison Reactor