University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Serum Cow

Classification: Biological fluid -> blood -> serum -> cow -> fetal

Citations 1

"A Microdialysis Fibre Based Sampler For Flow Injection Analysis: Determination Of L-lactate In Biofluids By An Electrochemically-synthesized Bilayer Membrane-based Biosensor"
Biosens. Bioelectron. 1996 Volume 11, Issue 4 Pages 419-425
F. Palmisano, D. Centonze, M. Quinto and P. G. Zambonin*

Abstract: A microdialysis sampler consisting of Spectra/por hollow-fiber regenerated cellulose (150 µm i.d; 9000 da mol. wt. cut-off) was inserted for ~2 cm in tygon tubing (10 cm x 0.5 mm i.d.) and sealed in. This sampler was used online for FIA to dilute the sample and remove surface active agents. FIA was carried out using a Gilson minipuls 3 pump, a six-way low pressure injection valve, a wall-jet flow-through cell, a PAR 400 detector and a Y-t chart recorder (schematic given). The sampler was coupled with a Pt/overoxidized polypyrrole/lactata oxidase/poly-o-phenylenediamine biosensor; preparation described previously (Anal. Chem.), 1995, 67, 1005). The enzyme electrodes were fitted to the wall-jet flow-through cell and left overnight at ± 0.7 V in 0.1 M phosphate buffer of pH 7 to complete poly (pyrrole) overoxidation. Calibration graphs were linear up to 10 mM lactate for standard lactate solutions. The method was applied to the analysis of bovine foetal serum; RSD was 1%. This technique can be used to analyze complex matrices such as serum, milk and yogurt with no sample pretreatment. A microdialysis fiber based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilized in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.
l-Lactate Sensor Electrode Electrode Electrode Electrode Interferences Hollow fiber membrane Dialysis