University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Biological tissue -> fish -> meat

Citations 2

"Biosensor For Detection Of Hypoxanthine Based On Xanthine Oxidase Immobilized On Chemically Modified Carbon Paste Electrode"
Anal. Chim. Acta 2000 Volume 412, Issue 1-2 Pages 55-61
Shengshui Hu, Cuilin Xu, Jiahui Luo, Jun Luo and Dafu Cui

Abstract: A novel hypoxanthine (Hx) sensor is constructed using a sodium montmorillonite-methyl viologen carbon paste modified electrode. Xanthine oxidase (XO) is immobilized within a polyaniline film on the electrode surface by electropolymerization. The hypoxanthine detection is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase, Electrocatalytic reduction of oxygen and the response of the sensor are evaluated using cyclic voltammetry. The response of the sensor is linear in the range of 1 µM-0.4 mM hypoxanthine and the minimum detectable level is 0.8 µM. The sensor can be applied to the determination of hypoxanthine in fish meat, and it is effective for eliminating interferences from coexisting substances in the samples.
Hypoxanthine Electrode Sensor Voltammetry Detector Interferences

"Flow-through Chemiluminescence Sensor Using Immobilized Histamine Oxidase From Arthrobacter Crystallopoietes KAIT-B-007 And Peroxidase For Selective Determination Of Histamine"
Anal. Sci. 2001 Volume 17, Issue 10 Pages 1161-1164
Yoshinori Sekiguchi, Aya Nishikawa, Hiroko Makita, Akira Yamamura, Kunio Matsumoto And Nobutoshi Kiba

Abstract: A flow sensor with immobilized oxidases is proposed for the determination of histamine in fish meat. Chemiluminometric measurement of histamine was based on the luminol reaction with hydrogen peroxide produced by immobilized histamine oxidase (EC 1.4.3.-.) and peroxidase (EC within a flow cell. Histamine oxidase was found in cells of Arthrobacter crystallopoietes KAIT-B-007 isolated from soil. The oxidase and peroxidase were coimmobilized covalently on tresylated hydrophilic vinyl polymer beads and packed into transparent PTFE; the tubing was used as the flow cell. One assay for histamine was done at intervals of 2 min without carryover. The calibration curve for histamine was linear from 0.1 µM to 50 µM. The response was reproducible within 1.25% of the relative standard deviation for 115-replicate injections of 50 µM histamine. The sensor system was applied to the determination of histamine in fish meat extracts.
Histamine Chemiluminescence Sensor Flowcell