University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Vegetable -> sugar beet

Citations 2

"Automatic Enzymatic Determination Of True Sucrose In Beet And Molasses"
Int. Sugar J. 1991 Volume 93, Issue 1110 Pages 121-125
Bengtsson, M.;Tjebbes, J.

Abstract: An outline is presented of a flow injection method in which the sample is injected into a stream of citrate buffer solution and passes through a 'glucose killer' reactor, containing immobilized aldose 1-epimerase, glucose oxidase and catalase, and then through a 'sucrose reactor' containing immobilized β-fructofuranosidase, aldose 1-epimerase and glucose oxidase. A color reagent is injected into the resulting stream to react with the H2O2 formed, and the product is detected colorimetrically. The method has been applied to digests of beet and to molasses and juices. Results were lower for cossettes than those obtained by the conventional pol analysis; the results for molasses differed only slightly from those of pol analysis and appeared to demonstrate that the correction usually applied for raffinose is too high.
Sucrose Automation Buffer Immobilized enzyme Reactor

"Determination Of Reducing Sugars Separated By High Performance Liquid Chromatography With Post-column Coloration"
Sucr. Fr. 1988 Volume 129, Issue NA Pages 117-124
Deruy, G.;Lescure, J.P.

Abstract: A mixture of reducing sugars (from, e.g., sugar beet, cane syrup or molasses) was analyzed on a column of Bio-Rad HPX 87 C cation-exchange resin (Ca(II) form) with aqueous Ca acetate as mobile phase and post-column reaction with 4-aminobenzohydrazide; detection was at 410 nm. Calibration graphs were rectilinear for 50 to 2000 mg L-1 of fructose, glucose, galactose or lactose. Up to 10 g L-1 of sucrose (or other non-reducing sugar) could be determined with use of a Bio-Rad HPX 87 H cation-exchange column (H+ form) after the separation column.
Sugars, reducing HPLC Spectrophotometry Post-column derivatization