University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Vegetable -> molasses

Citations 3

"Determination Of Sucrose In Sugar-cane Juice And Molasses By Flow Injection Spectrophotometry"
Anal. Chim. Acta 1988 Volume 204, Issue 1-2 Pages 259-270
E. A. G. Zagatto, I. L. Mattos and A. O. Jacintho

Abstract: The diluted and filtered sugar cane juice or molasses is introduced into a flow injection analyzer. with two merging streams, producing two sample zones. One zone is fed directly to the confluence of the streams but the other first flows through a heated coil where partial and reproducible sucrose (I) inversion is achieved at controlled pH and temperature At the confluence point a buffered IO4- reagent is added to oxidize I. The consumption of IO4- is measured spectrophotometrically as a transient lowering of the iodine concentration, produced by reaction of IO4- with I-. The two zones are fed sequentially to a flow cell where two peaks are recorded. The I concentration. is proportional to the difference in peak heights. About 30 samples h-1 of sugar-cane juice can be analyzed. The coefficient of variation was 0.51% for a I concentration. of 13.66% in cane juice (n = 7). Similar precision was obtained with the modified system for molasses (20 samples h-1).
Sucrose Spectrophotometry Indirect Merging zones Optimization Heated reaction

"Spectrophotometric Flow Injection Determination Of Sucrose And Total Reducing Sugar In Sugar-cane Juice And Molasses"
Anal. Chim. Acta 1988 Volume 214, Issue 1-2 Pages 247-257
I. L. Mattos, E. A. G. Zagatto and A. O. Jacintho

Abstract: Samples of sugar-cane juice or molasses were injected into the carrier stream (H2O at 1 mL min-1) which was then mixed with 1 M HCl in a 250-cm PTFE coil for hydrolysis of sucrose. This solution then merged with 3 M NaOH - 1%K3Fe(CN)6 reagent solution in a 580-cm PTFE coil, the solution was debubbled and 0.1% 1,10-phenanthroline - 0.5 M acetic acid - 0.1 M citric acid - 30 mg L-1 of Fe(III) was added. The solution passed through a 200-cm polyethylene coil and the absorbance was measured at 512 nm. The sampling rate for determination of sucrose and total reducing sugar was 40 h-1. The sampling rate was almost doubled when only reducing sugar was determined and a simplified flow system (illustrated) could be used. The coefficient of variation for determination of sucrose and total reducing sugar in sugar-cane juice were 0.47 and 0.38, respectively; those for molasses were 0.54 and 0.44%. Results generally agreed well with those from classical methods.
Sucrose Sugars, reducing, total Carbohydrates Spectrophotometry Debubbler Merging zones Method comparison

"Determination Of Reducing Sugars Separated By High Performance Liquid Chromatography With Post-column Coloration"
Sucr. Fr. 1988 Volume 129, Issue NA Pages 117-124
Deruy, G.;Lescure, J.P.

Abstract: A mixture of reducing sugars (from, e.g., sugar beet, cane syrup or molasses) was analyzed on a column of Bio-Rad HPX 87 C cation-exchange resin (Ca(II) form) with aqueous Ca acetate as mobile phase and post-column reaction with 4-aminobenzohydrazide; detection was at 410 nm. Calibration graphs were rectilinear for 50 to 2000 mg L-1 of fructose, glucose, galactose or lactose. Up to 10 g L-1 of sucrose (or other non-reducing sugar) could be determined with use of a Bio-Rad HPX 87 H cation-exchange column (H+ form) after the separation column.
Sugars, reducing HPLC Spectrophotometry Post-column derivatization