University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Vegetable -> broccoli

Citations 1

"Separation Of Ascorbic Acid, Isoascorbic Acid, Dehydroascorbic Acid And Dehydroisoascorbic Acid In Food And Animal Tissue"
J. Micronutr. Anal. 1990 Volume 7, Issue 1 Pages 67-70
Vanderslice, J.T.;Higgs, D.J.

Abstract: Samples (fresh broccoli and rat liver and kidney) were extracted with 8% acetic acid - 3% HPO3, the extracts were washed with hexane, with centrifugation, and the acid layers were analyzed on two PLRP-S (5 µm) columns (15 cm x 4.6 mm and 25 cm x 4.6 mm) in series operated at 4°C with 0.2 M NaH2PO4 (pH 2.14) as mobile phase (0.5 mL min-1). Post-column derivatization was carried out by oxidation with HgCl2 and subsequent reaction with o-phenylenediamine; detection was by fluorescence at 430 nm (excitation at 350 nm). Baseline separation of the cited analytes was achieved for both standards and sample extracts.
Ascorbic acid isoascorbic acid dehydroascorbic acid dehydroisoascorbic acid Fluorescence Column Post-column derivatization