University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Vegetable -> bean

Citations 3

"Rapid Determination Of Protein In Plant Material By Flow Injection Spectrophotometry With Trinitrobenzenesulfonic Acid"
Anal. Chim. Acta 1978 Volume 97, Issue 2 Pages 327-333
L. Sodek*, J. Rika and J. W. B. Stewart

Abstract: The rapid determination of protein in plant material based on spectrometric determination of amino acids in protein hydrolyzates with trinitrobenzenesulfonic acid was adapted to flow injection analysis With the manifold described, a routine sampling rate of 120 samples/h is possible, although this, as well as the sensitivity, can easily be varied. The method was calibrated against the Kjeldahl method and a good correlation was obtained between the 2 methods over a wide range of protein values for beans.
Amino Acids Protein Spectrophotometry Method comparison

"Flow Injection Flame Atomic Absorption Spectrometry For Slurry Atomization. Determination Of Calcium, Magnesium, Iron, Zinc And Manganese In Vegetables"
Anal. Chim. Acta 1993 Volume 283, Issue 1 Pages 393-400
P. Viñas, N. Campillo, I. López García and M. Hernández Córdoba*

Abstract: Washed cauliflower, bean, citrus and apple leaves were cut up, dried at 90°C, ground and calcined at 500°C for 1.5 h. The residue was ground and, for the determination of Fe, Mn and Zn with direct calibration, suspended in 10% glycerol solution containing 1% HCl, sonicated for 5 min and stirred for 10 min. The slurry was injected into a water carrier stream, which passed to the air-acetylene flame of the AAS instrument. The peak height of the transitory signal was measured. In the alternative standard addition method, the carrier was 10% glycerol/1% HCl containing added Mn(II), Fe(III) or Zn. For the determination of Ca and Mg, the ash was suspended in 1% HCl containing 1% La and 0.02% Triton X-100 and the slurry was injected into the online dilution device shown diagrammatically (Lopez Garcia et al., J. Anal. Atomic Spectrom., 1992, 7, 1291) with the same HCl-based solution as carrier. The determination limits were 12.4, 3.3 and 5.7 µg/g for Fe, Zn and Mn, respectively, and the corresponding RSD (n = 10) were 1.7, 0.9 and 0.8% at 57, 16 and 20 µg/g, respectively. In grapefruit leaves containing 0.4% Mg and 5.18% Ca, the RSD (n = 10) were 1.2 and 1.1%, respectively.
Calcium Magnesium Iron Zinc Manganese Spectrophotometry Slurry Triton X Standard additions calibration Surfactant

"Flow Injection Catalytic Spectrophotometric Determination Of Trace Manganese By Use Of The Reaction System Nitrilotriacetic Acid-manganese(II)-fuchsine-potassium Periodate"
Lihua Jianyan, Huaxue Fence 1994 Volume 30, Issue 2 Pages 86-88
Zi, Y.Q.;Chen, L.G.;Zeng, Q.G.

Abstract: Mn(II) solution (0.1 ml) was injected into the flow injection analyzer. to mix with a stream of 0.12 mM basic fuschine before further mixing and reaction with streams of 0.2 M acetic acid/0.2 M sodium acetate buffer of pH 4.5, 3 mM nitrilotriacetic acid and 15 µM-KIO4 (all streams at 2.52 ml/min) in a 130 cm reaction tube in a constant-temp. bath of 75°C, cooling in a 40 cm tube with running water as coolant and measurement of the decreased absorbance at 540 nm. The response was linear for up to 0.35 µg/ml, and the detection limit was 0.3 ng/ml. There was no interference in the determination of 0.1 µg/ml of Mn. The sampling frequency was 24 per h. The method was applied to environmental water, soil and cereals, including wheat and beans, with RSD of 1.3%.
Manganese Spectrophotometry Catalysis Heated reaction Interferences