University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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NIST 1549

Classification: Reference Material -> NIST -> 1549 -> Non-fat milk powder

Citations 1

"Optimization Of Selenium Determination In Human Milk And Whole Blood By Flow Injection Hydride Atomic Absorption Spectrometry"
J. AOAC Int. 1998 Volume 81, Issue 2 Pages 457-461
Amparo Alegría, Reyes Barberá, Rosaura Farré, Emilia Ferrer, M. Jesús Lagarda, M. Angeles Torres

Abstract: A flow injection hydride atomic absorption spectrometric (FI-HAAS) method was developed for determining selenium in human milk and whole blood after microwave digestion of the sample. The sample (2 mL human milk or 0.25 mL blood) was introduced into the microwave vessel with 1.5 mL HNO3 and 0.25 mL H2O2 and 300 W (4 min) and 600 W (4 min) were applied. The digestion was completed by heating to 140°C (2-3 h). Se(VI) was reduced to Se(IV) with HCl. The instrumental conditions for FI-HAAS (concentrations of reducing agent and carrier acid, flow rate of Ar carrier gas, and sample volume injected) were optimized. The detection limit of the proposed method was 0.23 ng/mL (assay) or 115 pg Se (abs.) in biological samples (1.15 ng/mL milk, 10.4 ng/mL blood). The precision values were 5.0% for milk and 4.0% for blood. The accuracy was evaluated with two NIST reference materials. Non-Fat milk powder (found: 104.3 ± 7.2 ng/g, certified: 110 ± 10 ng/g) and whole blood seronorm (found: 81 ± 7.3 ng/mL, ref.: 83 ± 4 ng/mL). The results show the suitability of the method for Se determination in human milk and whole blood. The method was applied to whole blood samples obtained from pregnant women and to human milk.
Selenium Spectrophotometry Sample preparation Reference material Optimization