University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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NIST 1508

Citations 1

"Certification Of Cocaine And Benzoylecgonine In A Human Urine Standard Reference Material"
J. Anal. Toxicol. 1992 Volume 16, Issue 3 Pages 158-162
Ellerbe P, Tai SS, Christensen RG, Espinosa-Leniz R, Paule RC, Sander LC, Sniegoski LT, Welch MJ, White E 5th

Abstract: Cocaine (I) was determined Standard Reference Material 1508 (freeze-dried human urine) by GC - MS and HPLC methods and benzoylecgonine (II) was determined by GC - MS and FIA - thermospray MS (details given). Concentrations were determined for three levels of I and three levels of II. After one year, the concentration. of I and II were determined again by GC - MS in order to investigate the stability of the standard; results showed no indication of any decomposition having occured. The precision of the measurements show that material is suitable for use as a control material and as an accuracy benchmark for the drug testing community. The concentrations of cocaine and benzoylecgonine (BE) in Standard Reference Material (SRM) 1508, cocaine and metabolites in freeze-dried human urine, were determined at the National Institute of Standards and Technology (NIST, formerly NBS) by two independent methods. For cocaine, one method was based on gas chromatography/mass spectrometry (GC/MS); the other was based on high performance liquid chromatography (HPLC). For BE, one method was based on GC/MS; the other was based on flow injection analysis/thermospray mass spectrometry (FIA/MS). The results for each pair of methods were statistically evaluated. Concentrations were determined in the SRM for three levels of cocaine and three levels of benzoylecgonine. Methylecgonine, although present in the material, was not determined. For cocaine, the concentrations were 90, 263, and 429 ng/mL of human urine. For BE, the concentrations were 103, 259, and 510 ng/mL of human urine.
Cocaine Benzoylecgonine Mass spectrometry Reference material Method comparison