University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Plant -> sugar beet -> leaves

Citations 1

"Comparative Study Of Post-column Reactions For The Detection Of Saccharides In Liquid Chromatography"
Anal. Chem. 1985 Volume 57, Issue 1 Pages 224-229
Petr Vratny, U. A. T. Brinkman, and R. W. Frei

Abstract: When 4-aminobenzohydrazide(I) was used as a post-column derivatization reagent, in conjunction with a Ca(II)-loaded cation-exchange column and water as mobile phase, 0.01 µg of saccharides could be detected. By inclusion of a solid-phase catalytic reactor (Anal. Abstr., 1985, 47, 3C10) between the HPLC column and the derivatization reactor, the method could be adapted for the determination of non-reducing sugars. Comparative studies with other post-column derivatization reagents [disodium bicinchoninate, 2-cyanoacetamide, tetrazolium blue and K3Fe(CN)6] showed I to be a more sensitive reagent. Application of I is illustrated by the analysis of sugars in an extract of sugar-beet leaves.
Saccharides HPLC Post-column derivatization