University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Plant -> soya bean -> leaves

Citations 1

"Determination Of Photosynthetic Intermediates In Leaves By High Performance Liquid Chromatography"
J. Chromatogr. A 1987 Volume 402, Issue 1 Pages 283-292
Shinichi Sawada*, Minobu Kasai, Tsuyoshi Yamada and Toyohiko Takahashi

Abstract: Sugar phosphates and nucleotides in extracts from leaves kept under photosynthetic conditions were separated on a column (25 cm x 6 mm, plus 1.5-cm pre-column) of strongly basic anion-exchange resin (Shodex TML, 15 to 20 µm) with programmed gradient elution by 0.6 M H3BO3 - 0.8 M NaH2PO4 buffer (pH ~4) during 3 h. The compounds in the eluate were subjected to reaction with 0.1% orcinol in 70% H2SO4 in a tube (47 m x 0.5 mm) heated in an oil bath at 130°C, followed by UV irradiation and detection of carbohydrates at 420 nm and of nucleotides at 260 nm. The method was applied particularly to samples from soya-bean leaves.
Nucleotides Sugars, phosphate HPLC Spectrophotometry Heated reaction Post-column derivatization