University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Plant

Classification: Plant -> pea

Citations 1

"Melittin-silica, A High-pressure Affinity Chromatography Resin For Calmodulin"
J. Chromatogr. A 1987 Volume 403, Issue 1 Pages 99-107
William S. Foster and Harry W. Jarrett

Abstract: The cited stationary phase for affinity chromatography of calmodulin(I) was prepared by coupling melittin to glycidyloxypropyl-silica. I was determined on a column (30 cm x 4.6 mm) of the cited resin with 10 mM sodium phosphate - 0.5 mM EGTA (pH 7.5) as mobile phase at 1 mL min-1 and detection at 230 nm. I was selectively bound in the presence of Ca(II) but not in its absence. With post-column derivatization with phthalaldehyde, <50 ng of I could be determined in brain or pea-plant extracts.
Calmodulin LC Sample preparation Spectrophotometry Post-column derivatization