University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Plant -> oilseed

Citations 1

"Liquid Chromatographic Determination Of Aflatoxins, Ochratoxin A And Zearalenone In Grains, Oilseeds And Animal Feeds By Post-column Derivatization And Online Sample Cleanup"
J. AOAC Int. 1989 Volume 72, Issue 2 Pages 336-341
Chamkasem N, Cobb WY, Latimer GW, Salinas C, Clement BA

Abstract: Cereal, oilseed or animal feed (50 g) was extracted with aqueous acetonitrile - KCl - H3PO4 for 1 h, and the extract was filtered, diluted with water, and analyzed (0.3 ml) on an Econosphere C18 (5 µm) column (15 cm x 4.6 mm) equipped with a cleanup pre-column (5 cm x 4.6 mm) of Adsorbosphere C18 (10 µm) and operated with gradient elution (details given) with 5 mM Na2HPO4 buffer (pH 3.7), methanol and acetonitrile at 1.5 mL min-1 and post-column derivatization with saturated aqueous iodine in a reaction coil at 90°C for fluorimetric detection at 425 nm (excitation at 360 nm). Calibration graphs were rectilinear for up to 300 ppb of aflatoxins B1, B2, G1 and G2 and ochratoxin A and up to 1000 ppb of zearalenone in the samples; the corresponding limits of determination were 5 and 30 ppb.
Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Ochratoxin A Zearalenone HPLC Fluorescence Sample preparation Post-column derivatization Buffer PPB