University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Plant -> groundnut -> meal

Citations 1

"Determination Of Aflatoxins In Groundnut Meal By High Performance Liquid-chromatography A Comparison Of Two Methods Of Derivatization Of Aflatoxin B-1"
Br. J. Biomed. Sci. 1995 Volume 52, Issue 4 Pages 312-316
Roch OG, Blunden G, Haig DJ, Coker RD, Gay C.

Abstract: A comparison has been made between pre-column and post-column derivatization of aflatoxin B-1 (AFB(1)) during estimation by high performance liquid chromatography of this toxin in groundnut meal. The effect of the use of different derivatization reagents on the quantification of aflatoxin B-2 (AFB(2)) has also been evaluated. Both AFB(1) and AFB(2) were analyzed at eight levels of artificial contamination. Five replicate analyzes were carried out at each level on both groundnut meal extract (acetone-water, 85:15, v/v) and extraction solvent alone. A statistical evaluation of the results gave limits of detection of 1.1 and 0.3 µg/kg for AFB(1) and AFB(2) respectively, using pre-column derivatization compared with 1.5 and 0.8 µg/kg for the post-column method. Recoveries of over 90% from the spiked groundnut meal extracts were achieved for both derivatization methods. (22 references)
Aflatoxin B1 HPLC Sample preparation Post-column derivatization Pre-column derivatization