University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Pharmaceutical -> patch

Citations 1

"Improvement On Simultaneous Determination Of Chromium Species In Aqueous-solution By Ion Chromatography And Chemiluminescence Detection"
Anal. Chim. Acta 1997 Volume 354, Issue 1-3 Pages 107-113
Bente Gammelgaarda,*, Yi-ping Liaob and Ole Jønsa

Abstract: A sensitive method for the simultaneous determination of chromium(III) and chromium(VI) was chromatography and chemiluminescence detection. Two Dionex ion-exchange guard columns in series, CG5 and AG7, were used to separate chromium(III) from chromium(VI). Chromium(VI) was reduced by potassium sulfite, whereupon both species were detected by use of the luminol-hydrogen peroxide chemiluminescence system. Parameters affecting retention times and resolution of the separator columns, such as eluent pH, eluent composition, reductant pH and concentration, and flow rates were optimized. Furthermore, the stabilities of reductant and luminol solutions were studied. The linear range of the calibration curve for chromium(III) and chromium(VI) was 1-400 µg L-1. The detection limit was 0.12 µg L-1 for chromium(III) and 0.09 µg L-1 for chromium(VI), respectively. The precision at the 20 µg L-1 level was 1.4% for chromium(III) and 2.5% for chromium(VI), respectively. The accuracy of the chromium(III) determination was determined by analysis of the NIST standard reference material 1643 c, Trace elements in water with the result 19.1±1.0 µg Cr(III) L-1 (certified value 19.0±0.6 µg Cr(III) l-1). The method was applied to analyze the stability of chromium patches for contact dermatitis testing. 19 References
Chromium(III) Chromium(VI) Chemiluminescence HPIC Detection limit Optimization Post-column derivatization Precision Preconcentration Speciation Reference material