University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

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Organic compound

Classification: Organic compound -> fatty acids

Citations 2

"Determination Of Iodine Value Of Fatty Acids By A Flow Injection Method"
Anal. Chim. Acta 1984 Volume 158, Issue 2 Pages 157-167
Cheng-chih Lee and Bruce D. Pollard

Abstract: A modification of the standard Wij solution method was used in an automated system controlled by an AIM-65 microcomputer. Iodine values of various industrial fatty acids were determined. Optimum operating conditions were established for the system, and a comparison was made between detection by u.v. absorbance and potentiometry. The coefficient of variation between observed and expected values were 9% for u.v. absorbance detection and 0.9% with potentiometric detection. Results obtained were similar to those of the conventional method. The max. continuous-run sample through-put was 12 h-1.
Iodine value Potentiometry Spectrophotometry Method comparison Optimization

"Enzymic Flow Injection Analysis For Essential Fatty Acids"
Sens. Actuat. B 1994 Volume 19, Issue 1-3 Pages 607-609
M. Schoemaker and F. Spener

Abstract: Solution (10 mM) of linoleic (I) and α-linolenic (II) acids in Tween 20 were prepared as described by Axelrod et al. (Methods in Enzymology, 1981, 71, 441). Portions (20 µL) were injected into the FIA system and were carried in a stream (450 µL/min) of 0.2 M potassium borate buffer of pH 9 containing 0.02% Tween 20, to a column (5 cm x 3 mm i.d.) of lipoxygenase immobilized on CNBr-activated Sepharose 4B. The decrease in O2 concentration resulting from enzyme-catalyzed lipid peroxidation was measured using a Clark-type O2 electrode held at -600 mV vs. Ag/AgCl. The calibration graphs for I and II were linear from 0.1-1.2 mM. The RSD for determination of 1 mM I (n = 55) was 1.4%.
Linoleic acid α-Linolenic acid Electrode Electrode Immobilized enzyme Sepharose beads Buffer