University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Oil -> coconut

Citations 2

"Determination Of Triglycerides By High Performance Liquid Chromatography With Post-column Derivatization"
Anal. Chem. 1986 Volume 58, Issue 12 Pages 2380-2383
Yukihiro Kondoh and Satoshi Takano

Abstract: The triglycerides are separated on two columns (15 cm x 4.6 mm) of Hitachi gel 3057 at 35°C with ethanol - acetonitrile (either isocratic or gradient elution) as mobile phase (0.8 mL min-1). The eluate is passed to a post-column reactor, where the triglycerides are hydrolyzed with 2.4% KOH in aqueous 60% ethanol and the resulting glycerol is oxidized at 70°C with 10 mM H5IO6 in ammonium acetate - acetic acid buffer (pH 5.5) to formaldehyde which is reacted with 0.2 M acetylacetone in the presence of ammonium acetate. The product is detected at 410 nm. Calibration graphs for trilaurin, trimyristin, tripalmitin and tristearin are rectilinear from 0.3 to 59.9, 0.3 to 50.7, 0.5 to 25.7 and 1.1 to 11.2 nmol, respectively, with coefficient of variation (n = 5) at the 5 µg level of <3.3%. The method has been applied to coconut oil.
Triglycerides Trilaurin Trimyristin Tripalmitin Tristearin HPLC Spectrophotometry Heated reaction Post-column derivatization

"Analysis Of Acylglycerols By High Performance Liquid Chromatography With Post-column Derivatization. 4. Simultaneous Analysis Of Mono-, Di- And Tri-acylglycerols"
J. Chromatogr. A 1987 Volume 393, Issue 3 Pages 427-432
Yukihiro Kondoh and Satoshi Takano

Abstract: The chromatograph and post-column reaction detector were as previously described (cf. Anal. Chem., 1986, 58, 2380). The mono-, di- and tri-glycerides, in acetone or THF solution, were separated by HPLC on a stainless-steel column (25 cm x 4 mm) of Hitachi gel 3057 (3 µm) operated at 30°C. The mobile phases were(I) ethanol - acetonitrile - water (10:12:3) and(II) ethanol - acetonitrile (13:7), at a flow rate of 0.8 mL min-1; initially, 100% of solvent(I) was used over 15 min and solvent(II) was increased by 4.67% min-1 to 70% and then by 1% min-1 to 100% during 30 min. Rectilinear response was obtained, e.g., for 1-monopalmitin in the range 0.2 to 121 nmol; the detection limit was 0.07 nmol. Results for standard mixtures, hydrogenated coconut oil - tallow oil mixture and a commercial emulsifier in a household product are shown.
Acylglycerols Monoglycerides Diglycerides Triglycerides HPLC Spectrophotometry Post-column derivatization