University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Marine -> invertibrate -> tissue

Citations 1

"High Performance Liquid Chromatographic Separation And Quantification Of Alanopine And Strombine In Crude Tissue Extracts"
Anal. Biochem. 1984 Volume 139, Issue 2 Pages 413-417
G. B. Fiore, C. V. Nicchitta and W. R. Ellington*

Abstract: Perchloric acid extracts of tissues of marine invertebrates were analyzed by HPLC on a Waters amino-acid column (25 cm x 4.6 mm) packed with strong cation exchanger. The mobile phase was 0.1 M citrate buffer (pH 3.0) at 0.4 mL min-1 and detection was by post-column derivatization with NaClO (at 60°C) and phthalaldehyde in the presence of 2-mercaptoethanol, with subsequent fluorimetry at 418 nm (excitation at 340 nm). Retention times of alanopine (2,2'-iminodipropionic acid) and strombine (2-methyliminodiacetic acid) were 4.7 and 5.4 min, respectively. Response was rectilinear from 0.05 to 0.25 nmol.
Alanopine Strombine HPLC Fluorescence Post-column derivatization Heated reaction