University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Whitefish Meal

Classification: Marine -> fish -> whitefish -> meal

Citations 1

"Selective Biosensing Of L-lysine By A Low-temperature Flow Injection Technique Using An Immobilized Lysine Oxidase Reactor"
Anal. Sci. 1996 Volume 12, Issue 1 Pages 87-90

Abstract: A method for the determination of L-lysine (lysine; I) using a H2O2 electrode as a biosensor device in an enzymatic FIA system is presented and used to analyze the concentration of I in fish feed. Squid or white-fish meal (10 g) was suspended in 100 mL 0.1 M phosphate buffer of pH 7.3 (buffer A) and the suspension was incubated with protease (10 000 U Denazyme AP) for a definite time. The reaction mixtures were incubated at 90°C for 10 min and centrifuged at 11 000 g for 1 h. The supernatants were filtered (0.45 µm) and a 20 µL portion of the filtrate was injected into a carrier stream of buffer A (3 ml/min) of an automated flow injection system (schematic shown). A computer-controlled switching valve downstream allowed the carrier to pass alternately through a lysine oxidase (LO)-immobilized aminopropyl-glass (APG, pore size 70 nm, 80-120 mesh) enzyme reactor and aa identical reactor containing APG only. The H2O2 (I) produced by the enzyme reactor was detected electrochemically at 10°C. The calibration graph was linear up to the mM range of I and the detection limit was of the order of tens of µM. The RSD (n = 8) was 1.45% for 0.5 mM I. Results agreed well with those obtained by HPLC.
l-Lysine Amperometry Electrochemical analysis Sensor Method comparison Immobilized enzyme Buffer Computer Glass beads Valve