University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Marine -> fish -> meal

Citations 2

"Phthalaldehyde Post-column Derivatization For The Determination Of Gizzerosine In Fish Meal By High Performance Liquid Chromatography"
J. Chromatogr. A 1990 Volume 515, Issue 1 Pages 527-530
Hiroyuki Murakita and Takeshi Gotoh

Abstract: Fish meal (200 mg) was hydrolyzed with 2 mL of 6 M HCl at 110°C for 22 h, and the digest was filtered and evaporated. The residue was dissolved in 2 mL of 10 mM phosphate buffer (pH 2.6) and cleaned up on a Bond Elut C18 cartrige. Analysis was on a column (15 cm x 4 mm) of Shim-pack ISC-07/S1504, with 30 mM sodium borate buffer (pH 9.8) at 45°C as mobile phase (0.4 mL min-1). The eluate was mixed with 15 mM citric acid containing 0.08% of phthalaldehyde and 0.4% of poly(oxyethylene lauryl ether) (0.2 mL min-1), and detection was by fluorimetry at 410 nm (excitation at 320 nm). The limit of detection was ~0.5 ppm, and the calibration graph was rectilinear for 1000 ng. Recovery of 10 ppm was 98.2%, with a coefficient of variation (n = 5) of 1.5%. The method should be useful, but an unknown interferent was observed.
Gizzerosine HPLC Post-column derivatization Buffer pH C18 Detection limit Calibration Interferences

"Determination Of Trace Platinum By Flow Injection Analysis - Adsorptive Stripping Voltammetry And Catalytic Polarographic Hydrogen Wave"
Fenxi Huaxue 1990 Volume 18, Issue 1 Pages 20-24
Wei Guizhen Lu Zongpeng* Alan M.Bond

Abstract: Platinum solution (0.1 µg mL-1; 100 µL) is injected into the flow injection analyzer. and reacts in a stream (0.27 mL min-1) of 0.002% hydrazine sulfate - 0.36 M H2SO4 and 0.04% formaldehyde - 0.36 M H2SO4. Detection is by adsorptive stripping voltammetry at -0.3 V for 60 s and measurement of the catalytic hydrogen wave at -0.8 V. Recoveries were 93.7 to 100% with a coefficient of variation of 5%. The calibration graph was rectilinear for 10 pg to 1 ng of Pt. Twenty samples can be run per hour. The method was applied in the analysis of urine, fish meal, milk powder, ox liver, minerals and organoplatinum compounds.
Platinum Voltammetry Polarography Catalysis Calibration