University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Industrial -> paper -> pulp

Citations 1

"Determination Of Anthraquinones In Pulping Materials By High Performance Liquid Chromatography Using Online Post-column Derivatization"
J. Chromatogr. A 1985 Volume 328, Issue 1 Pages 309-315
Nobutoshi Kiba, Motoharu Takamatsu and Motohisa Furusawa

Abstract: A selective assay for anthraquinones in black liquors and paper, based on their reduction by Na2S2O4 to anthraquinols, was developed. The anthraquinones were extracted with cyclohexane (from liquors) or CH2Cl2 (from paper) and portions of the extract were analyzed on a column (25 cm x 4.6 mm) packed with Jasco Finepak SIL C18 (10 µm), with acetonitrile - 0.3 mM HCl (7:3) as mobile phase (0.75 mL min-1) and mixing of the eluate with 0.2 M S2O42- in 0.5 M NaOH (at 0.5 mL min-1) and water (at 0.25 mL min-1), followed by passage of the mixture through a PTFE tube (100 cm x 0.5 mm) at 30°C and measurement of the absorbance at 500 nm. The method is applicable for 0.3 to 80 nmol of anthraquinones in 50 µL of injected sample. Mixtures of anthraquinone and four of its derivatives at ppm levels were analyzed by the proposed method, which is less sensitive than conventional HPLC methods.
Anthraquinone HPLC Spectrophotometry Heated reaction Post-column derivatization