University of North Florida
Browse the Citations

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

View Stuart Chalk's profile on LinkedIn


Classification: Fungus -> mycelia

Citations 1

"Detection Of Polyols And Sugars By Cuprammonium Ion In The Presence Of Strong Base"
Anal. Biochem. 1987 Volume 165, Issue 2 Pages 392-398
D. B. McKay, G. P. Tanner, D. J. Maclean* and K. J. Scott

Abstract: The cuprammonium reagent of Grimble et al. (Ibid., 1983, 128, 422) was modified so that final concentration. of active constituents in the reaction mixture were: 1.0 mM Cu(II), 0.4 M NH3 and 0.2 M NaOH (in excess); the absorbance (at 280 nm) of the modified reagent blank was <50% of that of previous methods. The new reagent was most sensitive for the colorimetric determination of polyols, especially D-glucitol, but it also produced a better color yield for some aldoses and disaccharides than the original reagent. It was used for post-column HPLC detection of various sugars and polyols; calibration graphs for D-glucitol, D-mannitol and D-glucose were rectilinear from 40 to 180 nmol, 0 to 150 nmol and 0 to 300 nmol, respectively. The method was applied in analysis of fungal mycelia.
Polyols Sugars d-Glucose d-Mannitol d-Glucitol HPLC Spectrophotometry Post-column derivatization