University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Fruit -> kiwi

Citations 1

"Flow Injection Determination Of Total Vitamin C Using Cucumber Juice Carrier"
Bunseki Kagaku 1990 Volume 39, Issue 11 Pages 793-795
Uchiyama, S.;Suzuki, S.

Abstract: The total ascorbic acid (I) content of lemons, mandarins and kiwi fruit was measured by a flow injection method after reduction of dehydroascorbic acid to L-ascorbic acid. Cucumber juice (filtered and centrifuged), containing ascorbate oxidase, was used as a carrier and the O consumed in the enzymatic reaction was detected at an O electrode. At a flow rate of 1.3 mL min-1 the peak currents were proportional to total I concentration. in the range 0.1 to 1 mM. The results were compared with those obtained by spectrophotometry. Ascorbate oxidase was stable in cucumber juice for 7 days.
Ascorbic acid Electrode Electrode Enzyme Method comparison