University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Fruit -> apple -> kernels

Citations 1

"Determination Of Cyanide In Microsamples By Means Of Capillary Flow Injection Analysis With Amperometric Detection"
Fresenius J. Anal. Chem. 1996 Volume 356, Issue 3-4 Pages 271-273
Ulli Backofen, Frank-Michael Matysik and G. Werner

Abstract: A new approach for determining cyanide in microsamples is described. The method is based on capillary flow injection analysis (CFIA) with amperometric detection. The sensing electrode is a silver-plated micro-disk electrode, where cyanide can react under formation of a dicyanoargentate complex. A remarkably low mass detection limit of 231 fmol cyanide is obtained for an injection volume of 60 nl. The sample throughput of the CFIA-arrangement is comparable with a conventional sized FIA-system. A practical application is given by analyzing the cyanide (amygdalin) concentration in apple kernels. The experimental set-up was described in detail. The detector consisted of a Pt microdisc electrode covered with a thin layer of silver. Measurements were made at a potential of 0 V vs. a Ag/AgCl reference electrode. The sample solution (60 nl) of standard cyanide (I) solution were injected into the carrier solution (640 nl/min) consisting of 50 mM borate buffer of pH 10.8. The calibration graph was linear from 3.84-384 µM-I with a detection limit of 0.384 µM. The method was used for the determination of amygdalin (AMY) in apple kernel indirectly by measuring I produced by the enzymatic hydrolysis of AMY with β-glucosidase.
Cyanide Amperometry Electrode Indirect Capillary