University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Fruit -> ackee

Citations 1

"Ion-exchange Chromatographic Determination Of Hypoglycine A In Canned Ackee Fruit"
J. AOAC Int. 1989 Volume 72, Issue 2 Pages 374-377
Chase, G.W.;Landen, W.O.;Gelbaum, L.T.;Soliman, A.G.M.

Abstract: Canned ackee fruit were drained from the liquid medium and homogenized with aqueous 80% ethanol, the homogenate was centrifuged and the supernatant solution was mixed with trichloroacetic acid. The mixture was filtered and a 15 µL portion was analyzed on a Waters automatic amino-acid analyzer. fitted with a No. 80002 ion-exchange column (25 cm x 4.6 mm) operated at 62°C with Na acetate buffer solution (pH 7.4) - Na citrate buffer solution (pH 3.15) (7:3) as mobile phase (0.4 mL min-1) and post-column derivatization with ninhydrin at 120°C for detection at 436 and 546 nm. Calibration graphs were rectilinear from 4.8 (detection limit) to 73 µg mL-1 of hypoglycine A in the final solution Recoveries of standard additions to the fruit and liquid medium (analyzed similarly) were 103 and 102%, respectively.
Hypoglycine A HPIC Spectrophotometry Filter Column Buffer Post-column derivatization Detection limit Standard additions calibration