University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Food -> syrup

Citations 2

"A Continuous Spectrophotometric System For The Discrimination/determination Of Monosaccharides And Oligosaccharides In Foods"
Anal. Chim. Acta 2000 Volume 404, Issue 1 Pages 121-129
A. Cáceres, S. Cárdenas, M. Gallego and M. Valcárcel

Abstract: A simple, rapid method for the spectrophotometric discrimination of monosaccharides from the oligosaccharide fraction of fruit juice, jam, syrup and honey samples is proposed. The sample, in alkaline medium, is directly introduced into a flow system and passed through an activated carbon column for its decolorization; then, a volume of 200 µl is injected into the derivatising reagent stream to start the analytical reaction, which takes place at 85°C. The two fractions are discriminated on the basis of the different colors of the derivatives formed; thus, the derivatives of the monosaccharides are yellow while those of the di- and trisaccharides are violet-carmine. The two fractions are monitored at 400 and 540 nm, respectively. Sucrose gives no reaction as it is a non-reducing sugar. The proposed method allows reducing sugars contents from 0.01% to 0.80% w/v to be determined with an average relative standard deviation of 4.5% and a sampling frequency of 10 h-1. The proposed method was validated by applying it to two milk-based and sugar candidate artificial CRMs, with good correlation. The detection limits achieved (0.01% w/v for maltose, lactose and maltotriose at 540 nm) allow adulteration of fruit juices with high fructose syrup from starch at the 4% level to be detected.
Monosaccharides Oligosaccharides Maltose Lactose Maltotriose Spectrophotometry Reference material Heated reaction Activated carbon Column

"Simultaneous Determination Of Sucrose And Reducing Sugars Using Indirect Flow Injection Biamperometry"
Anal. Chim. Acta 1993 Volume 271, Issue 2 Pages 239-246
Jacek Michawski and Anatol Koj, Marek Trojanowicz* and Bogdan Szostek, Elias A. G. Zagatto

Abstract: The cited method used a hexacyanoferrate(III) - hexacyanoferrate(II) indicating system. The flow injection system consisted of PTFE heating coils (0.7 mm), immersed in boiling water, and two injection valves. Two 150 µL portions of the sample were injected via the two valves with a time delay of 30 s. The first sample reacted with hexacyanoferrate(III); the second sample was hydrolyzed and then reacted with hexacyanoferrate(III). The resulting hexacyanoferrate(II) was detected. Changes in concentration. were monitored amperometrically with Pt wire electrodes polarized at 200 mV. Sucrose and glucose were determined with a sampling rate of 40 h-1. The method was applied to samples from white beet juice and syrups from stages of sugar production.
Sucrose Sugars, reducing Biamperometry Electrode Dual detection Indirect Heated reaction