University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Food -> garlic

Citations 3

"Direct And Individual Determination Of α-cyclodextrin, β-cyclodextrin And γ-cyclodextrin By Flow Injection Analysis Electrospray-ionization Mass-spectrometry"
Anal. Sci. 1997 Volume 13, Issue 5 Pages 815-816

Abstract: Liquid chromatography/mass spectrometry (LC/MS) has found wide applications as a reliable tool for the analysis of biological samples.(1-5) However, despite the fact that MS itself has the ability to identify and determine analytes in the presence of interfering substances, flow injection analysis/mass spectrometry (FIA/MS) has seldom been reported for the analysis of mixed components. Therefore, we attempted to demonstrate the excellent ability of FIA/MS in an individual determination of α-, β- and γ-cyclodextrins (CDs) in a mixture. The application of the established method to the analysis of commercial food products for β-CD is also reported. 5 References
α-Cyclodextrin β-Cyclodextrin γ-Cyclodextrin Mass spectrometry Interferences

"Determination Of Sulfoxides From Allium Species By Immobilized Alliinase"
Pharmaceut. Pharmacol. Lett. 1997 Volume 7, Issue 4 Pages 145-147
Krest, I.;Milka, P.;Keusgen, M.

Abstract: In recent years many attempts have been made to analyze the sulfur-containing constituents of garlic (Allium sativum L., Alliaceae) and related species by TLC and HPLC. These chromatographic techniques usually require a time consuming sample treatment including a pre-column derivatization for HPLC-analysis. In the present work, a number of analytical methods based on immobilized alliinase (EC was developed which allowed a rapid sample preparation as well as a high sample throughput. The alliinase was either integrated into a flow injection analyzer. (FIA) involving an ammonia detector or used for post-column derivatization combined with an HPLC separation and UV detection.
Sulfoxides HPLC Electrode Spectrophotometry Post-column derivatization Immobilized enzyme

"A High-throughput Method For The Quantitative Determination Of Alliin"
Planta Med. 1998 Volume 64, Issue 8 Pages 736-740
Michael Keusgen

Abstract: The quality of most garlic (Allium sativum) preparations made from garlic powder or garlic dry extract is determined by their content of alliin. Therefore, a comprehensive documentation of alliin concentration. beginning with the crude material up to the final remedy is required. The newly developed anal. method described in this paper was designed in order to fulfill these demands. In contrast to conventional HPLC methods, neither a pre-column derivatization nor a chromatography separation are involved in this anal. procedure allowing a high throughput of samples. The currently investigated technique is based on immobilized alliinase (EC which was combined with a two-channel flow injection analyzer (FIA) coupled to an ammonia detecting device. A high specificity for alliin could be demonstrated and a variety of garlic samples including garlic powders, dry extracts, and garlic preparations was analyzed. The results were in good correlation with those obtained by conventional HPLC methods.
Alliin Sensor Immobilized enzyme Method comparison