University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Food -> fish -> cube

Citations 1

"Spectrophotometric Simultaneous Determination Of Creatinine And Creatine By Flow Injection With Reagent Injection"
Fresenius J. Anal. Chem. 1995 Volume 352, Issue 6 Pages 557-561
Gloria del Campo, Ana Irastorza and J. Alfonso Casado

Abstract: Stock cubes (meat and fish) were dissolved in water by heating at 60°C with agitation and the resulting solution was cooled and filtered. After dilution with water a 220 µL portion was injected into the carrier stream of 2 g/l picric acid in 20 g/l NaOH in a FIA system. The system passed through a reaction coil (600 cm x 0.7 mm i.d.) and merged with 0.2 g/l of biacetyl and 25 g/l of 1-naphthol in 20 g/l of NaOH. The resulting stream passed through a second reaction coil (500 cm x 0.7 mm i.d.) to a spectrophotometer where the absorbance was measured at 520 nm. All flow rates were 0.9 ml/min and all reactions took place at 40°C. Calibration graphs were linear up to 30 mg/l of creatinine and creatine. RSD (n = 3) were 0.3-1.65% for 2-20 mg/l of creatinine and 0.34-0.67% for 2-30 mg/l of creatine. Recoveries were 99.6-102.9% for creatinine and 96.5-100.4% for creatine from meat and fish stock cubes.
Creatine Creatinine Spectrophotometry Heated reaction Reverse