University of North Florida
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Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Website: @unf

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Classification: Food -> coloring

Citations 1

"Isomeric Transformation Of 4-aminobiphenyl By UV Radiation And Its Influence On The Determination By Flow Injection Analysis With Amperometric And Spectrophotometric Detection"
Anal. Lett. 1995 Volume 28, Issue 15 Pages 2683-2697
Hernandez Blanco, M.;Quintana, M.C.;Hernandez, P.;Vicente, J.;Hernandez, L.

Abstract: Sample (200 µL) containing 4-aminobiphenyl (I; 0.1-1 µg/ml in methanol) was injected into a carrier stream (5.5 ml/min) containing 0.04 M Britton-Robinson buffer of pH 8 via a single-flow injection manifold. The streams were mixed in a reactor (20 cm x 0.5 mm i.d.) before amperometric detection in a wall-jet flow-cell with a working electrode potential of +0.9 V. Vitreous C, Ag/AgCl and Au were used as working, reference and auxiliary electrodes, respectively. Spectrophotometric measurements were performed similarly except that a 400 µL sample (containing 0.8-24 µg/ml of I in methanol) and a 0.02 M buffer of pH 10 were used. Detection was at 274 nm. For amperometric detection the calibration graph was linear from 0.1-1 µg/ml of I; quantitation and detection limits were 0.11 and 0.05 µg/ml, respectively. RSD were 2.5%. For the spectrophotometric method, the linear calibration range was 0.8-24 µg/ml of I; quantitation and detection limits were 0.4 and 0.05 µg/ml, respectively. RSD were 2.4%. Sample throughputs were 200 and 240 samples/h for amperometric and spectrophotometric detection, respectively. The methods were applied to the determination of I in food colorings. Two flow injection methods for the determination of 4-aminobiphenyl by amperometry (glassy carbon electrode) and spectrophotometry are proposed. A sample volume of 200 or 400 µl containing an analyte concentration of 0.1-1.0 µg mL-1 or 0.8-24.0 µg mL-1 for amperometric or spectrophotometric detection, respectively, is injected into a carrier stream containing 0.04 or 0.02 M Britton-Robinson buffer at pH 10.0 or 8.0, respectively. (11 References)
4-Aminobiphenyl Amperometry Electrode Spectrophotometry Photochemistry